文献类型：期刊文献

英文题名：Polyactin A and CpG enhance inactivated Pseudomonas plecoglossicida vaccine potency in large yellow croaker (Larimichthys crocea)

作者：Wang, Wenwen[1];Wang, Wei[2];Xu, Wenjun[2];Liu, Qin[1,3];Zhang, Yuanxing[1,3];Liu, Xiaohong[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Zhejiang Marine Aquat Res Inst, Key Lab Seawater Aquaculture, Zhoushan 316100, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2022

卷号：120

起止页码：133

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000724739600004)】；

基金：This work was financially supported by grants from Natural Science Foundation of Shanghai (21ZR1417300) , the second batch of Zhoushan city special funds for the development of modern fishery in 2020 (No. [2020] 21) , and the Fundamented Research Funds for Central Univer-sities (22221062017019) .

语种：英文

外文关键词：Inactivated vaccine; pCpG; Polyactin A; Pseudomonas plecoglossicida; Larimichthys crocea

摘要：Pseudomonas plecoglossicida is the causative agent of visceral granulomas disease (VGD) in large yellow croaker (LYC, Larimichthys crocea) farming. However, multi-antibiotic resistant of P. plecoglossicida creates an urgent need of an efficient vaccine to combat this pathogen. In this study, an inactivated vaccine added polyactin (PA), CpGriched plasmid (pCpG) and aluminum adjuvant (Al) was developed. As a result, its relative percentage survival (RPS) against P. plecoglossicida were up to 64%. Comparatively, RPS of groups that vaccinated with vaccines adjuvanted with PA and Al or CpG and Al were 49% and 39%. However, an interesting result that the vaccine combined with PA, CpG and Al did not show the strongest activation of total serum protein and antibody levels in serum among three vaccinated groups. According to expressions of some cellular immune related genes, we found that the inactivated vaccine combined with PA, CpG and Al was more likely to induce a cellular immune response rather than humoral immune response. Totally, our study demonstrated that the mixture of PA, CpG and aluminum adjuvant is a potential adjuvant system for LYC vaccine development.