文献类型：期刊文献

英文题名：Mitochondria-Targeted Ratiometric Fluorescent Probe Based on Diketopyrrolopyrrole for Detecting and Imaging of Endogenous Superoxide Anion in Vitro and in Vivo

作者：Wang, Jian[1,2];Liu, Lingyan[3];Xu, Weibo[4,5];Yang, Zhicheng[1,2];Yan, Yongchao[1,2];Xie, Xiaoxu[1,2];Wang, Yu[4,5];Yi, Tao[3];Wang, Chengyun[1,2];Hua, Jianli[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Fudan Univ, Dept Chem, 2005 Songhu Rd, Shanghai 200438, Peoples R China;[4]Fudan Univ, Shanghai Med Coll, Dept Oncol, Shanghai 200032, Peoples R China;[5]Fudan Univ, Shanghai Canc Ctr, Dept Head & Neck Surg, Shanghai 200032, Peoples R China

年份：2019

卷号：91

期号：9

起止页码：5786

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20192006912095);WOS:【SCI-EXPANDED(收录号:WOS:000467642100046)】；

基金：This work was supported by the National Natural Science Foundation of China (Grants 21772040, 21421004, 21788102, and 21572062), the Fundamental Research Funds for the Central Universities (Grant 222201717003), and the Programme of Introducing Talents of Discipline to Universities (Grant B16017). The authors thank Research Center of Analysis and Test of East China University of Science and Technology for help with the characterization.

语种：英文

外文关键词：Fluorescence - Ions - Probes - Mammals - Fluorescence spectroscopy

摘要：Intracellular reactive oxygen species is involved in a wide variety of physiological and pathological processes. In this work, we have developed a new mitochondria-targeting probe (DPP-S) for superoxide anion detection with ratiometric fluorescence response. DPP-S exhibited an obvious color change from violet to orange along with a distinct fluorescence change with maximum emission peak from 652 to 545 nm in response to superoxide anion. The limit of detection of DPP-S for superoxide anion was calculated to be 20.5 nM. Imaging studies taken in MCF-7 and RAW264.7 cells showed that DPP-S could be employed as a ratiometric fluorescent probe for endogenous superoxide anion detection and imaging in living cells with a large emission shift. Furthermore, the colocalization study indicated that DPP-S can localize in mitochondria specifically. Finally, the fluorescent probe was successfully applied for superoxide anion imaging in mice.