文献类型：期刊文献

英文题名：Microbiome changes and characteristics under nutrient injection for enhanced oil production at Daqing oilfield

作者：Fu, Zi-Yue[1,2,5];Chen, Wen-Tao[1,2,5];Qi, Gui-Na[1,2,5];Hou, Zhao-wei[3];Liu, Yi-Fan[1,2,5];Shou, Li-Bin[1,2,5];Zhou, Lei[1,2,5];Yang, Shi-Zhong[1,2,5];Wu, Xiao-Lin[3];Gu, Ji-Dong[4];Mu, Bo-Zhong[1,2,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]Daqing Oilfield Co Ltd, PetroChina, Explorat & Dev Res Inst, Daqing 163712, Heilongjiang, Peoples R China;[4]Guangdong Technion Israel Inst Technol, Environm Sci & Engn Res Grp, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China;[5]East China Univ Sci & Technol, MOE Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2025

卷号：196

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20244117179106);WOS:【SCI-EXPANDED(收录号:WOS:001335172100001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 42173076, 42061134011 and 52074129) , the

语种：英文

外文关键词：MEOR; JS1; Indigenous microbiome stimulation; Biosurfactant; Anaerobic hydrocarbon degradation

摘要：Current studies on Microbial Enhanced Oil Recovery (MEOR) mainly focus on introducing exogenous microbes or injecting biosurfactants into oil reservoirs, whereas simulating indigenous oil-displacing microorganisms has been less explored. In this study, we conducted MEOR applications on an oil reservoir in Daqing oilfield by injection of only nutrition solution (without microbial strains or biosurfactants), leading to an average increase of 218.6-221.4% in oil production. An alternative stable microbial community dominated by Pseudomonas species was established after MEOR applications, even one year after the termination of the injection. Additionally, members of Ca. Patescibacteria, Ca. Caldatribacteriota (JS1) and hydrogenotrophic methanogens Methanocorpusculum and Methanolinea were found as biomarkers for high oil productivity. These results demonstrate the potential to enhance crude oil production through biosurfactant production, acid and alcohol metabolites production, hydrocarbon degradation and biogas (CH4 and N2) production by the aforementioned stimulated indigenous oil-displacing microorganisms. This research provides theoretical guidance for the exploitation of depleted reservoirs and the extension of the development life of oil reservoirs.