文献类型：期刊文献

英文题名：A Single-Component Optogenetic System Allows Stringent Switch of Gene Expression in Yeast Cells

作者：Xu, Xiaopei[1,3];Du, Zhaoxia[1,3];Liu, Renmei[1,3];Li, Ting[1,3];Zhao, Yuzheng[1,2,3];Chen, Xianjun[1,2,3];Yang, Yi[1,3]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ct, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, CAS Ctr Excellence Brain Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China

年份：2018

卷号：7

期号：9

起止页码：2045

外文期刊名：ACS SYNTHETIC BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000445711300009)】；

基金：We thank Miaowei Mao, Zhengda Chen, Xie Li, Zengmin Du, Fangting Zuo and Ni Su for technical assistance. We thank Prof. Christof Taxis at Philipps-Universitat Marburg for providing sequence information on psd construct. We thank Prof. David Botstein at Princeton University for providing sequence information on GEV constructs. We thank Prof. Junbiao Dai at the Center for Synthetic Biology Engineering Research for providing TUB1/YML085C yeast strain. This research was supported by the National Key Research and Development Program of China (2017YFA050400 to Y.Y.), NSFC (31225008 and 31470833 to Y.Y., 31600688 to X.C.), the Shanghai Science and Technology Commission (18JC1411900, 14XD1401400, and 16430723100 to Y.Y.), China Postdoctoral Science Foundation (2016M59027 and 2017T00277 to X.C.), the State Key Laboratory of Bioreactor Engineering to Y.Y., the Fundamental Research Funds for the Central Universities to Y.Y. and X.C.

语种：英文

外文关键词：yeast; optogenetic; gene expression; protein stability

摘要：Light is a highly attractive actuator that allows spatiotemporal control of diverse cellular activities. In this study, we developed a single-component light-switchable gene expression system for yeast cells, termed yLightOn system. The yLightOn system is independent of exogenous cofactors, and exhibits more than a 500-fold ON/OFF ratio, extremely low leakage, fast expression kinetics, and high spatial resolution. We demonstrated the usefulness of the yLightOn system in regulating cell growth and cell cycle by stringently controlling the expression of His3 and (Delta N)Sicl genes, respectively. Furthermore, we engineered a bidirectional expression module that allows the simultaneous control of the expression of two genes by light. With ClpX and CIpP as the reporters, the fast, quantitative, and spatially specific degradation of ssrA-tagged protein was observed. We suggest that this single-component optogenetic system will be immensely helpful in understanding cellular gene regulatory networks and in the design of robust genetic circuits for synthetic biology.