文献类型：期刊文献

英文题名：Optimisation of the mRNA secondary structure to improve the expression of interleukin-24 (IL-24) in Escherichia coli

作者：Bai, Chaogang[1];Wang, Xiaojuan[1];Zhang, Jian[1];Sun, Aiyou[1];Wei, Dongzhi[1];Yang, Shengli[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：36

期号：8

起止页码：1711

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20142817917517);WOS:【SCI-EXPANDED(收录号:WOS:000339004900019)】；

基金：This work was supported by the grants from the National major science and technology projects of China (Grant No. 2012ZX09304009).

语种：英文

外文关键词：Anti-tumour; Interleukin 24; Melanoma; mRNA secondary structure; Translation initiation region

摘要：Interleukin-24 (IL-24) is a novel cytokine selectively inhibiting proliferation of cancer cells but with little effect on normal cells. However, IL-24 is difficult to express in Escherichia coli. In this study, we optimised the secondary structure of the translation initiation region using computational approach to obtain non-fusion recombinant IL-24 (nrIL-24). The Gibbs free energy of the region was decreased from -22 to -9.07 kcal mol(-1), potentially promoting a loose secondary structure formation and improving the translation initiation efficiency. As a result, the expression of nrIL-24 was increased to 26 % of the total cellular protein from being barely initially detectable. nrIL-24 showed a concentration-dependent inhibition of A375 cells but had little effect on normal human cells. These results demonstrate that this method in increasing nrIL-24 expression is effective and efficient.