文献类型：期刊文献

英文题名：Therapeutic efficacies of two newly isolated Edwardsiella phages against Edwardsiella piscicida infection

作者：Xu, Zhenhe[1];Shao, Shuai[1,3];Ding, Zihan[1];Zhang, Yuanxing[2,3];Wang, Qiyao[1,3];Liu, Xiaohong[1,3];Liu, Qin[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2022

卷号：263

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20222812347399);WOS:【SCI-EXPANDED(收录号:WOS:000829363800003)】；

基金：This work was supported by grants from National Natural Science Foundation of China, China (32025038) , Ministry of Agriculture of China, China (CARS -47) , and Shanghai Sailing Program, China (20YF1411100) .

语种：英文

外文关键词：Aquaculture; Cocktail; Edwardsiella piscicida; Phage therapy; Turbot; Zebrafish

摘要：The spread of multi-drug resistant (MDR) bacteria has posed a threat to the development of aquaculture. Due to its effective bactericidal ability, phage therapy has been considered as an alternative to antibiotics to reduce infection caused by MDR bacteria. In this study, two Edwardsiella piscicida phages were newly-isolated and characterized to prevent or treat infection in aquaculture. The phages were designated as vB_EpM_ZHS and vB_EpP_ZHX belonging to Myoviridae and Podoviridae families, respectively, in terms of genome sequence and morphology analyses. The combination of vB_EpM_ZHS and vB_EpP_ZHX improved the therapeutic efficacy both in vitro and in vivo. The phage cocktail significantly inhibited bacterial growth in vitro and decreased approximately 40% of mortality rate and an order of magnitude of bacterial burden in zebrafish and turbot infected by E. piscicida. Moreover, the phage cocktail increased transcription levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-12 (IL-12), interleukin-6 (IL-6), and interleukin-1 beta (IL-1 beta) and alleviated inflammatory levels in the hindgut and spleen of turbots. The results indicate that the phage has a promising potential for therapeutic use against E. piscicida as the antimicrobial alternative to antibiotics in aquaculture.