文献类型：期刊文献

中文题名：原花青素增强的聚乙二醇-溶菌酶生物活性水凝胶敷料的构筑及性能

英文题名：Fabrication and Performance of Proanthocyanidin Enhanced PEG-Lysozyme Bioactive Hydrogel Dressing

作者：陈占祎[1];谈浩琪[1];屈雪[1]

机构：[1]华东理工大学材料科学与工程学院,教育部医用生物材料工程研究中心,上海200237

年份：2021

卷号：34

期号：1

起止页码：74

中文期刊名：功能高分子学报

外文期刊名：Journal of Functional Polymers

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金(31922041);上海市科委科技创新项目(18441908300);中央高校基础研究基金(SLD 00202006,JKVD 12001032,JKVD 12001002,50321041916015)。

语种：中文

中文关键词：原花青素;力学增强;抗菌;抗炎;水凝胶敷料

外文关键词：proanthocyanidin;mechanical enhancement;antibacterial;anti-inflammatory;hydrogel dressing

摘要：以聚乙二醇(PEG)-溶菌酶(LZM)水凝胶(PEG-LZM)为模型,通过后浸泡法复合原花青素(PC)获得原花青素增强的聚乙二醇-溶菌酶水凝胶(PEG-LZM-PC)。通过扫描电子显微镜、傅里叶变换红外光谱仪和电子万能试验机考察了PEG-LZM-PC的形貌、结构和力学性能,通过平板涂布法评估了PEG-LZM-PC的抗菌能力,通过实时荧光定量聚合酶链式反应检测了PEGLZM-PC对免疫细胞炎症因子表达的影响。结果表明:PEG-LZM-PC的强度和韧性相比于PEGLZM有较大提升,且具有良好的体内外抗菌性能,并且可以抑制由脂多糖刺激引起的炎症反应,有望促进伤口愈合。
The proanthocyanidin(PC)-enhanced polyethylene glycol(PEG)-lysozyme(LZM)hydrogel(PEG-LZM-PC)was prepared based on PEG-LZM hydrogel by post-soaking with PC.The morphology,structure and mechanical properties of PEG-LZM-PC were characterized by scanning electron microscope(SEM),Fourier-transformed infrared(FT-IR)spectroscopy and universal testing instruments.Antibacterial ability of PEG-ZEM-PC was evaluated by plate coating method.The effect of PEG-LZM-PC on the expression of inflammatory factors in immune cells was evaluated by quantitative reverse transcription PCR(RT-qPCR).The results showed that the strength and toughness of PEG-LZM-PC were significantly improved compared with PEG-LZM;the antibacterial performance of PEG-LZM-PC in vivo and in vitro was pretty good;and the inflammatory response caused by lipopolysaccharide(LPS)stimulation was inhibited by PEG-LZM-PC,which is expected to promote wound healing.