文献类型：期刊文献

英文题名：Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes

作者：Lv, Yun[1];Cheng, Dan[1];Su, Dongdong[3];Chen, Mei[4];Yin, Bin-Cheng[2];Yuan, Lin[1];Zhang, Xiao-Bing[1]

机构：[1]Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China;[2]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]ASTAR, Lab Bioimaging Probe Dev, Singapore Bioimaging Consortium, 11 Biopolis Way,Helios 02-02, Singapore 138667, Singapore;[4]Hunan Univ, Coll Mat Sci & Engn, Changsha 410082, Hunan, Peoples R China

年份：2018

卷号：9

期号：39

起止页码：7606

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20184205964824);WOS:【SCI-EXPANDED(收录号:WOS:000448407200015)】；

基金：This work was financially supported by the NSFC (21622504, 21735001, and 21877029), the Science and Technology Project of Hunan Province (2017RS3019), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. All animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Hunan University and experiments were approved by the Animal Ethics Committee of College of Biology (Hunan University).

语种：英文

外文关键词：Fluorescence spectroscopy - Infrared devices - Diagnosis - Fluorescence

摘要：Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O-2(center dot-)), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O-2(center dot-) with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes (Naph-O-2(center dot-) and NIR-O-2(center dot-) ) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O-2(center dot-) in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O-2(center dot-) level during the drug-induced nephrotoxicity. Moreover, probe NIR-O-2(center dot-) was also used to elucidate the protective effects of L-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O-2(center dot-) in complex nephrotoxicity disease systems.