文献类型：期刊文献

英文题名：Effects of Pb(II) exposure on Chlorella protothecoides and Chlorella vulgaris growth, malondialdehyde, and photosynthesis-related gene transcription

作者：Xiong, Bang[1,2]; Zhang, Wei[1,2]; Chen, Lin[1,2]; Lin, Kuang-Fei[1,2]; Guo, Mei-Jin[3]; Wang, Wei-Liang[3]; Cui, Xin-Hong[4]; Bi, Hua-Song[4]; Wang, Bin[4]

机构：[1] State Env. Protection Key Lab. of Env. Risk Ass. and Control on Chemical Process, East China University of Science and Technology, Shanghai, 200237, China; [2] School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China; [3] The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; [4] Shanghai Institute of Landscape Gardening, Shanghai, 200233, China

年份：2020

卷号：29

期号：11

起止页码：1346

外文期刊名：Environmental Toxicology

收录：EI(收录号：20143600053394)

语种：英文

外文关键词：Polymerase chain reaction - Aldehydes - Aquatic organisms - Plants (botany) - Transcription - Lead compounds

摘要：Greater exposure to Pb(II) increases the likelihood of harmful effects in the environment. In this study, the aquatic unicellular alga Chlorella protothecoides (C. protothecoides) and Chlorella vulgaris (C. vulgaris) were chosen to assess the acute and chronic toxicity of Pb(II) exposure. Results of the observations show dose-response relationships could be clearly observed between Pb(II) concentration and percentage inhibition (PI). Exposure to Pb(II) increased malondialdehyde (MDA) content by up to 4.22 times compared with the control, suggesting that there was some oxidative damage. ANOVA analysis shows that Pb(II) decreased chlorophyll (chl) content, indicating marked concentration-dependent relationships, and the lowest levels of chl a, chl b, and total-chl were 14.53, 18.80, and 17.95% of the controls, respectively. A real-time PCR assay suggests the changes in transcript abundances of three photosynthetic-related genes. After 120 h exposure Pb(II) reduced the transcript abundance of rbcL, psaB, and psbC, and the relative abundances of the three genes of C. protothecoides and C. vulgaris in response to Pb(II) were 54.66-98.59, 51.68-95.59, 37.89-95.48, 36.04-94.94, 41.19-91.20, and 58.75-96.80% of those of the controls, respectively. As for 28 d treatments, the three genes displayed similar inhibitory trend. This research provides a basic understanding of Pb(II) toxicity to aquatic organisms. ? 2013 Wiley Periodicals, Inc.