文献类型：期刊文献

英文题名：Graphene Oxide as a Matrix for Enzyme Immobilization

作者：Zhang, Jiali[2];Zhang, Feng[1];Yang, Haijun[2];Huang, Xuelei[1];Liu, Hui[1];Zhang, Jingyan[1];Guo, Shouwu[2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Natl Key Lab Micro Nano Fabricat Technol, Res Inst Micro Nano Sci & Technol, Shanghai 200240, Peoples R China

年份：2010

卷号：26

期号：9

起止页码：6083

外文期刊名：LANGMUIR

收录：;EI(收录号：20101812910769);WOS:【SCI-EXPANDED(收录号:WOS:000276969700002)】；

基金：This work was supported by the National "973 Program" (Nos. 2007CB936000 and 2010CB933900) and the NSFC of China (Nos. 20774029 and 20671034).

语种：英文

外文关键词：Atomic force microscopy - Molecules - Catalyst activity - Graphene - Surface treatment

摘要：Graphene oxide (GO), having a large specific surface area and abundant functional groups, provides an ideal substrate for study enzyme immobilization. We demonstrated that the enzyme immobilization on the GO sheets could take place readily without using any cross-lit king reagents and additional surface modification. The atomically flat surface enabled us to observe the immobilized enzyme in the native state directly using atomic force microscopy (AFM). Combining the AFM imaging results of the immobilized enzyme molecules and their catalytic activity, we illustrated that the conformation of the immobilized enzyme is mainly determined by interactions of enzyme molecules with the functional groups of GO.