文献类型：期刊文献

英文题名：Comparative transcriptome analysis reveals the redirection of metabolic flux from cell growth to astaxanthin biosynthesis in Yarrowia lipolytica

作者：Wang, Dan-Ni[1];Yu, Chen-Xi[1];Feng, Jie[1];Wei, Liu-Jing[1];Chen, Jun[1];Liu, Zhijie[2,3];Ouyang, Liming[1];Zhang, Lixin[1,4];Liu, Feng[1,5];Hua, Qiang[1,4,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Minist Educ & Hubei Prov, Minist Educ, Cooperat Innovat Ctr Ind Fermentat, Key Lab Fermentat Engn, Wuhan, Peoples R China;[3]Hubei Univ Technol, Hubei Key Lab Ind Microbiol, Wuhan, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai, Peoples R China;[5]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2024

卷号：41

期号：6

起止页码：369

外文期刊名：YEAST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001201760900001)】；

基金：This study was supported by the National Key R&D Program of China (2017YFE0115600, 2019YFA0904302), and the National Natural Science Fund for Young Scholars (32001035).

语种：英文

外文关键词：astaxanthin; atmospheric and room-temperature plasma mutagenesis; membrane stress; RNA-seq analysis; Yarrowia lipolytica

摘要：Engineering Yarrowia lipolytica to produce astaxanthin provides a promising route. Here, Y. lipolytica M2 producing a titer of 181 mg/L astaxanthin was isolated by iterative atmospheric and room-temperature plasma mutagenesis and diphenylamine-mediated screening. Interestingly, a negative correlation was observed between cell biomass and astaxanthin production. To reveal the underlying mechanism, RNA-seq analysis of transcriptional changes was performed in high producer M2 and reference strain M1, and a total of 1379 differentially expressed genes were obtained. Data analysis revealed that carbon flux was elevated through lipid metabolism, acetyl-CoA and mevalonate supply, but restrained through central carbon metabolism in strain M2. Moreover, upregulation of other pathways such as ATP-binding cassette transporter and thiamine pyrophosphate possibly provided more cofactors for carotenoid hydroxylase and relieved cell membrane stress caused by astaxanthin insertion. These results suggest that balancing cell growth and astaxanthin production may be important to promote efficient biosynthesis of astaxanthin in Y. lipolytica.