文献类型：期刊文献

英文题名：Effect of adaptive laboratory evolution of engineered Escherichia coli in acetate on the biosynthesis of succinic acid from glucose in two-stage cultivation

作者：Jiang, Jiaping[1];Luo, Yuanchan[1];Fei, Peng[1];Zhu, Zhengtong[1];Peng, Jing[1];Lu, Juefeng[1];Zhu, Du[4];Wu, Hui[1,2,3]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Dalian Univ Technol, Sch Bioengn, MOE Key Lab Biointelligent Mfg, Dalian, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Jiangxi Sci & Technol Normal Univ, Coll Life Sci, Key Lab Bioproc Engn Jiangxi Prov, Nanchang 330013, Peoples R China

年份：2024

卷号：11

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20243717022112);WOS:【SCI-EXPANDED(收录号:WOS:001197740200001)】；

基金：No Statement Available

语种：英文

外文关键词：Adaptive laboratory evolution; E. coli; Acetate; Succinic acid; Two-stage fermentation

摘要：Escherichia coli MLB (MG1655 Delta pflB Delta ldhA), which can hardly grow on glucose with little succinate accumulation under anaerobic conditions. Two-stage fermentation is a fermentation in which the first stage is used for cell growth and the second stage is used for product production. The ability of glucose consumption and succinate production of MLB under anaerobic conditions can be improved significantly by using acetate as the solo carbon source under aerobic condition during the two-stage fermentation. Then, the adaptive laboratory evolution (ALE) of growing on acetate was applied here. We assumed that the activities of succinate production related enzymes might be further improved in this study. E. coli MLB46-05 evolved from MLB and it had an improved growth phenotype on acetate. Interestingly, in MLB46-05, the yield and tolerance of succinic acid in the anaerobic condition of two-stage fermentation were improved significantly. According to transcriptome analysis, upregulation of the glyoxylate cycle and the activity of stress regulatory factors are the possible reasons for the elevated yield. And the increased tolerance to acetate made it more tolerant to high concentrations of glucose and succinate. Finally, strain MLB46-05 produced 111 g/L of succinic acid with a product yield of 0.74 g/g glucose.