文献类型：期刊文献

英文题名：Fungicidal Effect of Pyraclostrobin against Botrytis cinerea in Relation to Its Crystal Structure

作者：Xiong, Hui[1];Liu, Xuefeng[1];Xu, Jiuyong[1];Zhang, Xianfei[1];Luan, Shaorong[2];Huang, Qingchun[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2020

卷号：68

期号：39

起止页码：10975

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20204509462712);WOS:【SCI-EXPANDED(收录号:WOS:000577116200049)】；

基金：This work was financially supported by the grants from the Project in Shanghai Science and Technology Innovation Action Plan of Shanghai, China (19391902400 and 15431906800).

语种：英文

外文关键词：pyraclostrobin; XRPD; crystal structure; mycelium growth; conidial germination; germ tube elongation; mitochondrial metabolites; Botrytis cinerea

摘要：Pyraclostrobin (PYR) is a commonly used strobilurin fungicide, which inhibits mitochondrial respiration at the ubiquinol oxidation center site of the cytochrome bc1 complex. Little information is available regarding the crystal structure of PYR on its fungicidal effect. In this study, the crystal structures of eight PYRs (PYR-A to H) from different sources are determined by using high-resolution X-ray powder diffraction (XRPD) and model construction with the Pawley refinement module. The effects of PYRs on mycelium growth, the kinetics of mycelial growth, conidial germination, and tube elongation of conidia of Botrytis cinerea from tomato are compared. The level of organic acids in the mitochondrial tricarboxylic acid cycle of PYR-treated B. cinerea is analyzed. The results show that PYR-A to PYR-H have their own unique character of XRPD patterns, but the crystal morphology of eight PYRs presents in the triclinic crystal system and space group P (1) over bar. PYR-D with the eclipsed conformation and rational edge angles alpha (72.599 degrees) and beta (98.612 degrees) in the crystal cell shows the highest inhibitory effect against mycelium growth with EC50 as 3.383 mu g mL(-1), the best time-dependent effects on the mycelium growth kinetics, and the strongest inhibition on tube elongation of conidia, whereas PYR-E with anticonformation is the worst. Moreover, a significant accumulation of fumarate, malate, and oxalate in the PYR-D-treated mycelium is observed. These findings reinforce the need for a definite crystal structure of PYR to limit usage and mitigate future selection pressure for gray mold management.