文献类型：期刊文献

中文题名：CO2对脱氮假单胞菌发酵生产维生素B12的影响

英文题名：Effects of Carbon Dioxide on Cell Growth and Vitamin B_(12) Production in Pseudomonas Denitrificans

作者：王慧媛[1];王泽建[1];黄明志[1];储炬[1];庄英萍[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心,上海200237

年份：2010

卷号：36

期号：4

起止页码：499

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家863计划(2006AA02Z204)

语种：中文

中文关键词：溶解CO2;脱氮假单胞菌;维生素B12

外文关键词：dissolved CO2 ; Pseudomonas denitrificans; vitamin B12

摘要：通过调整进气中空气和CO2的比例,研究了CO2的体积分数(φCO2)为0.03%,3.32%,8.86%和13.84%时对脱氮假单胞菌的生长和维生素B12合成代谢的影响。发酵过程的生理代谢分析结果表明:当进气中CO2体积分数增加到8.86%时,菌体的生长没有受到显著影响;而单位菌体糖消耗速率为29.95 mg/(g.h),仅为对照组的77%;产物的合成速率可达到1.75 mg/(L.h),比对照组1.02 mg/(L.h)的合成速率提高了71.6%。但CO2体积分数为13.84%时,菌体生长、底物利用以及维生素B12的合成都将受到抑制,因此在生产过程中控制CO2的体积分数非常关键。
The effects of CO2 concentrations on cell growth and vitamin B12 biosynthesis metabolism of Pseudomonas denitrificans were investigated by controlling the inlet gas （containing air and COz） at the adjusted φco2 of 0.03%, 3. a2%, 8.86% and 13.84%. The fermentation physiological metabolism analysis results showed that hardly any significant influence on cell growth was observed when φco2 increased to 8.86%. When φco2=8.86%, the specific glucose consumption rate decreased to 29.95 mg/（g.h）, only 77% of that of the control, and the specific vitamin B12 production rate reached 1.75 mg/（L.h）, 71.6% higher than that of the control. However, when the inlet φco2 increased to 13.84%, cell growth, substrate consumption, and vitamin B12 production were greatly inhibited. Therefore, it is important for controlling the φco2 in industrial vitamin El2 fermentation.