文献类型：期刊文献

英文题名：Selective profiling of liver-related specific proteins based on sofosbuvir-modified magnetic separation material

作者：Pan, Yini[1];Wang, Zhenxin[2];Xu, Sen[1];Zhang, Lingyi[1];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai, Peoples R China;[2]Fudan Univ, Zhongshan Hosp, Dept Lab Med, Shanghai, Peoples R China

年份：2023

卷号：39

期号：3

起止页码：313

外文期刊名：ANALYTICAL SCIENCES

收录：;EI(收录号：20225213309165);WOS:【SCI-EXPANDED(收录号:WOS:000903992900001)】；

基金：Financial support was from National Natural Science Foundation of China (21974045, 82102503), the National Key R&D Program of China (2021YFF0701900), the Science and Technology Commission of Shanghai Municipality (No. 19142201100, 6142200500), the Fundamental Research Funds for the Central Universities (No. JKJ01221718), and Zhongshan Hospital Fudan University Youth Foundation (2020ZSQN30).

语种：英文

外文关键词：Sofosbuvir; Protein profiling; Liver diseases; Separation material; Proteomics

摘要：It has great significance in profiling specific proteins throughout for better understanding of complex pathological processes and in-depth pharmacological studies. In this work, an efficient protein profiling strategy was developed based on the specific protein-drug interaction. Sofosbuvir (SOF), as a first-line drug for the treatment of hepatitis C, was modified onto the surface of nanoparticles through stable chemical bonds to fabricate a novel magnetic separation material denoted as Fe3O4@SiO2@PAA@SOF. With sequence coverage as the screening parameter, nine proteins were profiled from fetal bovine serum (FBS) of which eight were liver related. Similarly, the strategy was applied to hepatocellular carcinoma (HCC) patient serum. Eight proteins were profiled and all of them were liver related, demonstrating the superb specificity and selectivity of this strategy for profiling liver-related proteins by virtue of protein-SOF interaction. When serum proteins from HCC patients were compared to those from healthy people, one unique differential protein (D3DQX7) was profiled, which was liver related and was a potential target for ameliorating liver diseases. For further research, this material design concept and protein profiling strategy can be extended to employ other drugs for corresponding studies.