文献类型：期刊文献

英文题名：Multiplexed detection of microRNAs by tuning DNA-scaffolded silver nanoclusters

作者：Zhang, Min[1];Liu, Yu-Qiang[1];Yu, Cui-Yuan[1];Yin, Bin-Cheng[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2013

卷号：138

期号：17

起止页码：4812

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000322389600017)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (Grants no. 21205040, 21075040), the Key Grant Project of Chinese Ministry of Education (no. 313019), the Shanghai 12ZR1442700, 11XD1401900, and 11nm0502500, the Fundamental Research Funds for the Central Universities, and Hitachi, Ltd.

语种：英文

摘要：A universal silver-nanocluster method coupled with target-triggered isothermal exponential amplification reaction (TIEAR) is developed for light-up fluorescent detection of multiple microRNAs (miRNAs) in a label-free format. Taking advantage of the interesting feature of the fine-tuned emission spectrum of fluorescent DNA-scaffolded silver nanoclusters (DNA/AgNCs), our proposed assay is designed such that the different miRNA targets are transferred to the different oligonucleotide reporters via the TIEAR, in which unimolecular DNAs designed for different targets are employed as the amplification templates, polymerases and nicking enzymes as mechanical activators and miRNA targets as the trigger. The produced oligonucleotide reporters act as templates for the synthesis of multicolor DNA/AgNC probes, which correspond to different target inputs. This proposed method has been well validated on the multiplex detection of miRNAs and DNAs, as well as in practical application.