文献类型：期刊文献

英文题名：Efficient heterotrophic cultivation of Chlamydomonas reinhardtii

作者：Zhang, Zhen[1];Tan, Yingying[1];Wang, Weiliang[1];Bai, Wenmin[2];Fan, Jianhua[1];Huang, Jianke[1];Wan, Minxi[1];Li, Yuanguang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,301 Mail Box, Shanghai 200237, Peoples R China;[2]Jiaxing Zeyuan Bioprod Co Ltd, Jiaxing, Zhejiang, Peoples R China

年份：2019

卷号：31

期号：3

起止页码：1545

外文期刊名：JOURNAL OF APPLIED PHYCOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000469393600004)】；

基金：This work was partially supported in finance by the National Natural Science Foundation of China (NSFC; 21706071) and China Postdoctoral Science Foundation (2018M632044).

语种：英文

外文关键词：Chlamydomonas reinhardtii; Chlorophyceae; Heterotrophy; Scale-up; Cultivation

摘要：With a unique cell structure and clear genetic background, Chlamydomonas reinhardtii has become a model species for fundamental research on microalgae and lower plants. Genetic transformations in the nucleus and chloroplast and mitochondrial genomes of C. reinhardtii have been successfully developed. However, research of C. reinhardtii has mainly focused on physiology and molecular genetics and few studies investigating cultivation methods have been performed. Small scales, slow growth, and low yields from conventional photoautotrophic and mixotrophic cultures severely limit the further development and application of C. reinhardtii. This study sought to optimize and scale up the heterotrophic cultivation process for C. reinhardtii. Critical parameters in 500-mL shake flasks were optimized as follows: liquid volume of 150mL, initial inoculation of 0.08gL(-1), growth temperature of 30 degrees C, and shaking speed of 150rpm. In addition, this study confirmed that, although C. reinhardtii can assimilate acetic acid for heterotrophic growth (optimum initial concentration of 80mM), neither glucose nor glycerol can be the only carbon source. Nitrate, ammonia, and urea can be used as nitrogen sources for heterotrophic culture, while the optimal carbon and nitrogen ratio for heterotrophic culture was 40:1. Under optimal conditions, the C. reinhardtii biomass increased from 0.457 to 1.32gL(-1). Furthermore, for the first time, heterotrophic cultures of C. reinhardtii were scaled up to 5-L and 50-L fermenters, in which the cell density reached 25.44gL(-1) after 237h, which is 2.82-fold higher than previously reported.