文献类型：期刊文献

英文题名：Neutrophil plays critical role during Edwardsiella piscicida immersion infection in zebrafish larvae

作者：Wang, Zhuang[1];Lin, Lingyun[2];Chen, Weijie[1];Zheng, Xin[1];Zhang, Yuanxing[1,3];Liu, Qin[1,3];Yang, Dahai[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Inst Freshwater Fisheries, Huzhou 313001, Peoples R China;[3]Shanghai Engn Res Ctr Marine Cultured Anim Vaccin, Shanghai 200237, Peoples R China

年份：2019

卷号：87

起止页码：565

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000464086600061)】；

基金：This work was supported by the National Natural Science Foundation of China No. 31602187 (D.Y.) and the Fundamental Research Funds for the Central Universities No. 222201714022 (D.Y.). Dahai Yang was supported by the Young Elite Scientists Sponsorship Program by CAST No. 2016QNRC001, Shanghai Pujiang Program No.16PJD020, Shanghai Chenguang Program No.16CG33, and the Talent Program of the Fast China University of Science and Technology, Shanghai, China.

语种：英文

外文关键词：Edwardsiella piscicida; Zebrafish larvae; Neutrophil; Bacterial clearance

摘要：Edwardsiella piscicida is a facultative intracellular pathogen that causes hemorrhagic septicemia and haemolytic ascites disease in aquaculture fish. During bacterial infection, macrophages and neutrophils are the first line of host innate immune system. However, the role of neutrophils in response to E. piscicida infection in vivo remains poorly understood. Here, through developing an immersion infection model in the 5 day-post fertilization (dpf) zebrafish larvae, we found that E. piscicida was mainly colonized in intestine, and resulted into significant pathological changes in paraffin sections. Moreover, a dynamic up-regulation of inflammatory cytokines (TNF-alpha, IL-1 beta, GCSFb, CXCL8 and MMP9) was detected in zebrafish larvae during E. piscicida infection. Furthermore, a significant recruitment of neutrophils was observed during the E. piscicida infection in Tg(mpx:eGFP) zebrafish larvae. Thus, we utilized the CRISPR/Cas9 system to generate the neutrophil-knockdown (gcsfr(-/-) crispants) larvae, and found a comparative higher mortality and bacterial colonization in gcsfr(-/-) crispants, which reveals the critical role of fish neutrophils in bacterial clearance. Taken together, our results developed an effective E. piscicida immersion challenge model in zebrafish larvae to clarify the dynamic of bacterial infection in vivo, which would provide a better understanding of the action about innate immune cells during infection.