文献类型：期刊文献

中文题名：葡萄糖氧化酶复合物电极的研制

英文题名：A Stable Enzyme Biosensor for Determination of Glucose

作者：叶帮策[1];李友荣[1]

机构：[1]华东化工学院生化工程系

年份：1993

卷号：19

期号：4

起止页码：445

中文期刊名：华东化工学院学报

收录：国家哲学社会科学学术期刊数据库;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：电极;葡萄糖;氧化酶

外文关键词：biosensor; enzyme electrode; concanavalin A; thermal stability; glucose oxidase

摘要：研究了葡萄糖氧化酶(GoD)和伴刀豆球蛋白A(ConA)复合物的热稳定性,计算了其在不同温度下的热失活常数k_d。实验结果表明,GOD-ConA复合物与GOD相比,可显著提高酶的热稳定性。应用该复合物制作的二茂铁媒介生物传感器,在室温22℃条件下,响应电流在10d后基本没有衰减。
The thermal stability of insoluble ConA complex of glucose oxidase was researched. The thermal deactivation rate constants of the complexes were obtained. It was found that the GOD-ConA complexes are less sensitive to thermal inactivation than the native enzyme. By using the complexes, the ferrocene-mediated enzyme electrodes were constructed. The results suggested that the electrodes have good thermal stability at room temperature (22℃).