文献类型：期刊文献

英文题名：Separation of Piper kadsura Using Preparative Supercritical Fluid Chromatography Combined with Preparative Reversed-Phase Liquid Chromatography

作者：Xin, Huaxia[1];Dai, Zhuoshun[1];Cai, Jianfeng[1];Ke, Yanxiong[1];Feng, Jiatao[2];Fu, Qing[1];Jin, Yu[1];Liang, Xinmiao[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China

年份：2018

卷号：81

期号：8

起止页码：1181

外文期刊名：CHROMATOGRAPHIA

收录：;EI(收录号：20242616503470);WOS:【SCI-EXPANDED(收录号:WOS:000439334900006)】；

基金：This study was funded by the Project of National Natural Science Foundation of China (81403100) and Fundamental Research Funds for the Central Universities (222201717016).

语种：英文

外文关键词：Supercritical fluid chromatography; Reversed-phase liquid chromatography; Purification; Natural products; Piper kadsura

摘要：We have developed a separation strategy that employed preparative supercritical fluid chromatography (prep-SFC) combined with preparative reversed-phase liquid chromatography (prep-RPLC) to purify compounds from Piper kadsura. The complexity of the sample was effectively reduced by two steps of prep-SFC. The key experimental parameters of prep-SFC were systematically optimized, including an "average-pressure rule" scaling-up method for adjustment of the backpressure of the system. A mixed modifier consisting of ethanol/n-hexane was applied to provide a stable elution. Prep-RPLC was subsequently adopted to purify the SFC fractions due to its good orthogonality with SFC. Furthermore, chiral SFC was used to separate the structural analogues. As a result, 14 compounds with high purity were obtained from one fraction, including three pairs of diastereoisomers. These data indicate that the separation strategy using prep-SFC combined with prep-RPLC is an effective tool to separate complex samples such as natural products.