文献类型：期刊文献

中文题名：脂肽的分离纯化与结构研究

英文题名：Isolation and Identification of a Lipopeptide

作者：吕应年[1];杨世忠[1];牟伯中[1]

机构：[1]华东理工大学化学系,上海200237

年份：2005

卷号：32

期号：1

起止页码：67

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目(No.59974014);高等学校博士学科点专项科研基金资助课题(No.20030251002);上海市教委资助项目~~

语种：中文

中文关键词：枯草芽孢杆菌;脂肽;分离纯化;环状结构

外文关键词：Bacillus subtilis, Lipopeptide, Isolation, Cyclic structure

摘要：采用酸沉淀分离、有机溶剂提取、分级沉淀、脱色吸附及制备薄板的方法,从枯草芽孢杆菌HSO121的培养液中分离得到一种脂肽类化合物。茚三酮试验、红外光谱分析表明该脂肽具有环状结构,电喷雾离子化质谱结果显示,该脂肽是分子量为1022D和1036D的两个同系物,氨基酸分析及串联质谱分析表明,脂肽的肽链结构为Leu-Leu-AspValLeu-Leu-Glu,说明它是Surfactin的结构类似物。
A lipopeptide compound was isolated from the culture of Bacillus subtilis HSO121 by methods of acidic precipitation, solvent extract, fractional precipitation, adsorption and prepared thin-layer chromatography; and its molecular structure was determined by by ninhydrin assay and IR methods following the Amino analysis, MS-MS and ESI-MS. It shows that the isolated lipopeptide consists of two homologues with molecular mass 1,022D and 1,036D and bearing a cyclic structure with the amino acid sequence Leu-Leu-Asp-Val-Leu-Leu-Glu in the peptide chain, which indicates that the isolated lipopeptide falls into the analogs of surfactin.