文献类型：期刊文献

英文题名：Manipulation of ginsenoside heterogeneity in cell cultures of Panax notoginseng by addition of jasmonates

作者：Wang, W; Zhong, JJ

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2002

卷号：93

期号：1

起止页码：48

外文期刊名：JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000174399400009)】；

语种：英文

外文关键词：Panax notoginseng; plant cell culture; ginsenoside; heterogeneity of plant secondary metabolites; methyljasmonate; traditional Chinese medicinal herb

摘要：Manipulation of the heterogeneity of ginsenosides with different pharmacological activities was attempted by adding methyl jasmonate (MJA) or dihydro-methyl jasmonate (HMJA) to suspension cultures of a high-saponin-producing cell line of Panax notoginseng. Compared with HMJA, MJA not only increased ginsenoside content to a large degree but also significantly altered the ratio of Rb to Rg groups of ginsenosides. The ginsenoside Rd was not detected in either control cultures (without MJA addition) or when HMJA was added, but interestingly was detected upon supplementation of MJA. By increasing the concentration of MJA added from 0 to 500 muM, the ratio of Rb to Rg groups also increased from 0.49 +/- 0.06 to 2.49 +/- 0.06 (on day 15). The cells exhibited their maximal ginsenoside content when 200 muM MJA was added. The maximal Rb, content was 3.15 +/- 0.08 mg/100 mg DW (day 15), which was about ninefold that of the control. The maximal Rg, and Re contents were 0.90 +/- 0.01 (day 12) and 0.80 +/- 0.01 (day 15) mg/100 mg DNA, respectively, which were about twofold that of the control. The information obtained will be useful for the exploitation of the diversity of biopharmaceutical molecules and for the large-scale bioprocessing of heterogeneous products.