文献类型：期刊文献

中文题名：含环氧基的交联聚合物载体的制备方法对固定化青霉素酰化酶活性的影响

英文题名：Influence of Preparation Methods of Copolymer Supports Containing Oxirane Groups on Activity of Immobilized Penicillin Acylase

作者：薛屏[1];卢冠忠[1];郭耘[1];郭杨龙[1];王筠松[1]

机构：[1]华东理工大学工业催化研究所

年份：2003

卷号：24

期号：10

起止页码：733

中文期刊名：催化学报

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：环氧基;聚合物载体;固定化青霉素酰化酶

外文关键词：oxirane group, copolymer support, immobilized penicillin acylase

摘要：以甲基丙烯酸缩水甘油酯为单体 ,N ,N′ 亚甲基双丙烯酰胺为交联剂 ,采用两种方法合成了大孔、珠状的交联聚合物固定化酶载体 .用红外光谱、扫描电子显微镜及N2 吸附等方法测定了其结构、比表面积、孔径分布和表观活性 .结果表明 ,以液体石蜡为主介质、甲醇水溶液为致孔剂合成的聚合物GM1( 6 0 )作载体时 ,固定化酶水解青霉素G的活性达 5 37U/ g;以正庚烷与四氯乙烯混合溶剂为介质、甲酰胺为致孔剂合成的聚合物GM 2 ( 6 0 )作载体时 ,固定化酶的活性较低 ,为 4 2 6U/g .在 37℃下连续进行 10次间歇操作 (每次反应 10min)后 ,前者活性降至 4 87U/ g,保持了初始活性的 90 7% ;后者活性降至 378U/ g,保持了初始活性的 88 7% .二者催化活性的不同是由于两种方法制备的载体在结构与性能上存在着明显的差异 .GM1( 6 0 )载体孔径大 ,水中溶胀性能好 ,对青霉素酰化酶的偶联作用强 ,固定化效果显著 .
The immobilized penicillin acylase has played an important role in the manufacture of semi synthetic antibiotics. Much effort is being made to develop new types of carriers to improve the catalytic efficiency of the enzyme and reduce the cost of the supports. Oxirane groups of copolymer supports can couple to amino groups of enzymes under mild conditions so that the enzyme molecules can be immobilized on the surface of the supports. In this work, the immobilization of penicillin acylase on a copolymer support containing oxirane groups is reported. The macroporous beads of the copolymer support containing oxirane groups was synthesized by two methods with glycidyl methacrylate as the reactive monomer and N,N′ methylene bis acrylamide as the crosslinking agent. The support structure, specific surface area, and pore size distribution were characterized by Fourier transform infrared spectroscopy, scanning electron microscope, and N 2 adsorption. The results show that the effect of the preparation method on the properties of the copolymer is very great. The GM1(60) copolymer, which was prepared using paraffin oil as the main polymerization medium and aqueous methanol as the pore forming agent, has higher specific surface area, larger pore diameter, and better swelling capacity in water. The GM2(60) copolymer, which was prepared in a mixed solvent of n heptane and using tetrachloroethylene as the suspending medium and formamide as the pore generating agent, gives a lower capacity to couple enzyme. The activity of GM1(60) immobilized penicillin acylase and GM2(60) immobilized penicillin acylase is 537 and 426 U/g , respectively. The former decreases to 487 U/g and the latter decreases to 378 U/g after the immobilized enzymes are used for the consecutive hydrolysis of penicillin G at 37 ℃ for 10 times (10 min each time). Evidently, the GM1(60) copolymer is superior to GM2(60) as the support for immobilization of penicillin acylase. The GM1(60) copolymer has higher capacity to couple penicillin acylase and the enzyme immobilized on GM1(60) keeps higher activity during the operation than that on GM2(60).