文献类型：期刊文献

中文题名：菌群降解菲和氧化三价砷的双功能验证

英文题名：Functional Verification of A Dual-functional Consortium:Degrading Phenanthrene and Oxidizing Arsenite Simultaneously

作者：汤军芝[1];冯天才[1];刘世友[1];冯耀宇[1]

机构：[1]华东理工大学资源与环境工程学院国家环境保护化工过程风险评价与控制重点实验室,上海200237

年份：2013

卷号：39

期号：4

起止页码：450

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

语种：中文

中文关键词：菲;砷;复合污染;功能基因;多样性;实时荧光定量PCR

外文关键词：phenanthrene; arsenic; combined pollution; diversity; real-time quantitative PCR

摘要：从上海某含菲和砷复合污染物的焦化厂土壤中富集、分离得到了一个双功能菌群。在含200mg／L菲和60mg／L As2O3的培养基中，该菌群48h内能降解71．4％的菲和氧化96．2％的三价砷。从分子水平进行功能验证，选择菲降解基因（RHDa）和砷氧化基因（aoxB）构建文库，基因RHDa与Pseudomonas aeruginosa和Pseudomonas putida基因的同源性较高；而基因“aoxB与Pseudomonas stutzeri和Alcaligenes faecalis的同源性较高。采用实时荧光定量PCR技术定量2个基因，在48h内，1mL菌液中aoxB基因拷贝数为3．00×10^8～1．48×10^10，而RHDa则为2．54×10^7～8．70×10^9。该菌群将在含多环芳烃和砷复合污染场地中具有潜在的应用价值。
A heterotrophic bacterial consortium, degrading Phenanthrene（PHE） and oxidizing As（ Ⅲ ） simultaneously, was isolated from the soil in a discarded coking plant containing PHE and As（Ⅲ ）. After 48 b incubation, the bacterial consortium removed 71.4% of PHE and transformed 96.2% of As（ Ⅲ ） in liquid culture containing 200 mg/L PHE and 60 mg/L As203. To validate the dual-function of the consortium from molecular level, phenanthrene-degrading gene RHDa and arsenite-oxidizing gene aoJB were chosed to establish clone libraries. The RHDa genes had high homology with Pseudomonas aeruginosa and Pseudomonas putida, while aoxB genes had high homology with Pseudomonas stutzeri and Alcaligenes faecalis. Real-time quantitative PCR results showed that the copy numbers of aoxB genes increased from 3.00× 10^8 to 1.48× 10^10, while the copy numbers of RHDa genes increased from 2.54×10^7 to 8. 70 × 10^10 after 48 h incubation. The dual function consortium showed potentials for practical applications of degrading phenanthrene and arsenic co contamination.