文献类型：期刊文献

中文题名：限域石英纳米孔道实时监测点击化学反应

英文题名：Real-Time Monitoring of Click Reaction Based on Confined Glass Nanopipette

作者：芦思珉[1];于汝佳[1];龙亿涛[1,2]

机构：[1]华东理工大学化学与分子工程学院,上海200237;[2]南京大学化学化工学院生命分析化学国家重点实验室,南京210023

年份：2019

卷号：47

期号：9

起止页码：1309

中文期刊名：分析化学

外文期刊名：Chinese Journal of Analytical Chemistry

收录：CSTPCD;;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000487218200006)】；北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21834001).

语种：中文

中文关键词：点击化学反应;限域石英纳米孔道;电化学限域效应;体积排阻效应;阻断电流

外文关键词：Click reaction;Confined glass nanopipette;Electrochemically confined effect;Volume exclusion effect;Ionic blockage

摘要：点击化学反应具有反应条件温和、高区域选择性等优点,被广泛应用于材料科学、药物科学、生物化学等研究领域。在单颗粒水平上,实时监测点击化学反应过程有助于揭示反应的各向异性及反应机理。本研究以具有电化学限域效应的石英纳米孔道作为传感器,对单个金纳米颗粒(AuNPs)上的点击化学反应进行了实时动态监测。在电渗流的驱动下,炔基化合物修饰的AuNPs进入孔道内部,在Cu+的催化下,AuNPs表面的炔基与金层上的叠氮基团发生点击化学反应,因此,AuNPs在孔道端部的滞留时间增长。相比于未发生点击化学反应时,反应过后“体积排阻效应”造成的阻断电流频率变大,阻断时间明显增长。因此,基于限域石英纳米孔道的分析方法为在单颗粒水平上实时动态监测化学反应提供了新的研究思路。
Click reaction is a reliable,powerful and selective method in material science,medical science and biochemistry.Monitoring of click reaction at single particle scale contributes to revealing anisotropy and mechanism of click reaction.In this study,electrochemically confined glass nanopipette was acted as a sensor to monitor the click reaction on a single gold nanoparticle(Au NP).Driven by electroosmotic flow,Au NPs modified with alkynyl moved towards the inner channel of glass nanopipette.When Cu+was presented in the inner channel,alkynyl on the surface of Au NPs would react with azide on the gold layer.Click reaction resulted in the cross-linking between Au NPs and gold layer,which hindering the movement of Au NPs.Hence,Au NPs were transitorily resided near the orifice of glass nanopipette,which was responded to a higher frequency of ionic blockage and a longer blockage time.The method proposed here paved a way for real-time monitoring of chemical reaction.