文献类型：期刊文献

英文题名：Dual-mode exosome detection leveraging a nanozyme-active artificial receptor: PDA@Fe@Zn-based nucleic acid aptamer sensor

作者：Zhao, Linghao[1];Kuang, Jingjing[1];Xiang, Keheng[1];Gan, Jiayue[1];Zeng, Yuxin[2];Zhang, Xichen[1];Yan, Yicheng[1];Zhang, Min[2];Zhang, Hongyang[1];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai 200237, Peoples R China

年份：2025

卷号：285

外文期刊名：TALANTA

收录：;EI(收录号：20245217567864);WOS:【SCI-EXPANDED(收录号:WOS:001393101400001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant number 81973285) . The authors are grateful to Shi-Yi Zhang for her assistance and thank the Research Centre of Analysis and Test of East China University of Science and Technology for help.

语种：英文

外文关键词：Exosome detection; Polydopamine; Nanozymes; Dual-mode sensor; Signal amplification

摘要：Exosomes, extracellular vesicles crucial for intercellular communication, are emerging as significant biomarkers for disease diagnosis, especially in cancer. This study presented a dual-mode exosome detection platform using polydopamine microspheres doped with iron and zinc ions (PDA@Fe@Zn). These materials served as both artificial receptors for nucleic acid aptamers and nanozymes with peroxidase-like activity. By integrating colorimetric and fluorescence detection, the platform enables cross-validation of results. PDA@Fe@Zn nanozymes catalyzed the TMB-H2O2 reaction under acidic conditions, producing a colorimetric signal proportional to exosome concentration. Concurrently, the fluorescence of FAM-labeled aptamers was dynamically quenched by PDA@Fe@Zn and the presence of exosomes restores the fluorescence signal for a "turn-on" detection mode. DNase I amplified detection signals by cleaving bound exosomes for multiple cycles, achieving a limit of detection (LOD) of 4.7 x 104 particles/mL for colorimetric detection and 2.2 x 104 particles/mL for fluorescence detection. Notably, the colorimetric platform revealed that the relative expression of the CD63 protein on exosomes from breast cancer cells MCF-7 and MDA-MB-231 was approximately 2.7 and 2.4 times higher, respectively, than in normal breast cells MCF-10A; similar fold changes of 2.9 and 2.4 were observed with fluorescence detection, underscoring the robustness of the dual-mode system. The platform demonstrated rapid detection (within 30 min), high sensitivity, strong anti-interference capability, and the ability to distinguish exosomes from cancerous and normal cells effectively.