文献类型：期刊文献

英文题名：A rapid-response near-infrared fluorescent probe with a large Stokes shift for senescence-associated β-galactosidase activity detection and imaging of senescent cells

作者：Qiu, Wenjing[1];Li, Xiaokang[1];Shi, Donglei[1];Li, Xinming[1];Gao, Ying[2];Li, Jinwen[1];Mao, Fei[1];Guo, Yuan[2];Li, Jian[1,3,4]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]Northwest Univ, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol, Minist Educ, Xian 710127, Peoples R China;[3]Dali Univ, Coll Pharm & Chem, Inst Mat Med, Dali 671000, Peoples R China;[4]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai 200237, Peoples R China

年份：2020

卷号：182

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20203209011751);WOS:【SCI-EXPANDED(收录号:WOS:000564524200002)】；

基金：This work was supported by the National Key R&D Program of China (2017YFB0202600), the National Natural Science Foundation of China (81872747, 21977082, 81903457), the Innovative Research Team of High-level Local Universities in Shanghai, the Natural Science Basic Research Plan for Distinguished Young Scholars in Shaanxi Province of China (2020JC-38), the National Special Fund for State Key Laboratory of Bioreactor Engineering, China (2060204), the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, China (2018OPEN12), the Chinese Postdoctoral Science Foundation, China (2018M641946), the Shanghai Sailing Program, China (19YF1412600), and the Shanghai Morning Light Program, China (18CG33).

语种：英文

外文关键词：Senescence-associated beta-galactosidase; NIR fluorescent probe; Aging; Senescent cells; Living-cell imaging

摘要：Detection of senescence-associated beta-galactosidase (SA-beta-gal) activity at an increased lysosomal pH is one of the most reliable approaches for probing senescent cells. Herein, we developed a novel near-infrared fluorescent probe (KSL0401) for sensing SA-beta-gal activity and applying in visualization of aging. This probe was designed by connecting a cleavable D-galactose residue to the coumarin-dicyanoisophorone hybrid fluorophore KSL0401-OH via a beta-glycosidic bond. The spectral properties of KSL0401 reaction with beta-gal were measured in aqueous solution at pH 6.0, and a remarkable enhancement of fluorescence emission at 706 nm was observed, along with a visible colorimetric change. Probe KSL0401 was highly selective and sensitive to SA-beta-gal, and displayed large Stokes shift (218 nm) and good biocompatibility. Moreover, it exhibited extremely rapid response to beta-gal within 80 s, which surpassed most of the reported SA-beta-gal probes. Additionally, KSL0401 also enabled to detect the endogenous SA-beta-gal activity in living cells, and distinguish between senescent cells and non-senescent cells and qualify the degree of aging. These findings demonstrate that KSL0401 is a potential detection tool in the imaging research of aging.