文献类型：期刊文献

中文题名：Spatiotemporal Imaging of Cellular Energy Metabolism with Ge- netically-Encoded Fluorescent Sensors in Brain

英文题名：Spatiotemporal Imaging of Cellular Energy Metabolism with Ge- netically-Encoded Fluorescent Sensors in Brain

作者：Zhuo Zhang[1,3];Weicai Chen[1,3];Yuzheng Zhao[1,3];Yi Yang[1,2]

机构：[1]Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, Shanghai Collabora- tive Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, Shanghai 200237, China;[2]Optogenetics and Synthetic Biology Interdisciplinary Research Center, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;[3]Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

年份：2018

卷号：34

期号：5

起止页码：875

中文期刊名：神经科学通报：英文版

外文期刊名：Neuroscience Bulletin

收录：CSTPCD;;Scopus;CSCD:【CSCD2017_2018】；PubMed;

基金：This review was supported by the National Key Research and Development Program of China (2017YFA050400 and 2017YFC0906900), the National Natural Science Foundation of China (31722033, 91649123, 31671484, 31225008, and 31470833), the Shanghai Science and Technology Commission (14XD1401400, 16430723100, and 15YF1402600), Young Elite Scientists Sponsorship Program by China Association for Science and Technology (to YZ), Shanghai Young Top-notch Talent (to YZ), the State Key Laboratory of Bioreactor Engineering (to YY), and Fundamental Research Funds for the Central Universities (to YY and YZ).

语种：中文

中文关键词：星形细胞;新陈代谢;传感器;荧光灯;精力;大脑;编码;成像

外文关键词：Energy metabolism;Astrocyte;Neuron;Genetically encoded fluorescent sensor;Real time monitoring

摘要：大脑有很高的精力要求并且在人的身体消费 20% 氧和 25% 葡萄糖。因此，位于下面大脑怎么使物质产生代射变化支持神经活动的分子的机制是为神经科学研究的一个基本问题。在大脑的一个著名模型，星形细胞神经原喂奶梭，要求那葡萄糖举起和 glycolytic 活动在 neuronal 激活和那星形细胞运输之上在星形细胞被提高喂奶进神经原完成他们的精力要求。为这个假设的当前的证据还得到达清楚的一致，并且在梭假设以外的新概念是新兴的。差异大部分为在细胞的分辨率的新陈代谢的动力学的即时监视被归因于一个批评方法的缺乏。最近的进展在荧光灯基于蛋白质的传感器允许 subcellular 代谢物的敏感、特定的、即时读出的产生并且充满当前的工艺的差距。这里，我们在在生活房间并且在 vivo 估计房间新陈代谢总结遗传上编码的代谢物传感器和他们的应用的发展，并且我们相信这些工具将帮助阐明的问题到地址神经精力新陈代谢。
The brain has very high energy requirements and consumes 20% of the oxygen and 25% of the glucose in the human body. Therefore, the molecular mechanism under- lying how the brain metabolizes substances to support neural activity is a fundamental issue for neuroscience studies. A well-known model in the brain, the astrocyte- neuron lactate shuttle, postulates that glucose uptake and glycolytic activity are enhanced in astrocytes upon neu- ronal activation and that astrocytes transport lactate into neurons to fulfill their energy requirements. Current evidence for this hypothesis has yet to reach a clear consensus, and new concepts beyond the shuttle hypothesis are emerging. The discrepancy is largely attributed to the lack of a critical method for real-time monitoring of metabolic dynamics at cellular resolution. Recent advances in fluorescent protein-based sensors allow the generation of a sensitive, specific, real-time readout of subcellular metabolites and fill the current technological gap. Here,we summarize the development of genetically encoded metabolite sensors and their applications in assessing cell metabolism in living cells and in vivo, and we believe that these tools will help to address the issue of elucidating neural energy metabolism.