文献类型：期刊文献

英文题名：Enhancement of Sulfur Conversion Rate in the Production of L-Cysteine by Engineered Escherichia coli

作者：Liu, Han[1];Hou, Yehua[1];Wang, Yu[1];Li, Zhimin[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：68

期号：1

起止页码：250

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20200208022587);WOS:【SCI-EXPANDED(收录号:WOS:000507147100026)】；

基金：This study was supported by the China National Key Research and Development Program (Grant No. 2019YFA0904300) and the Fundamental Research Funds for the Central Universities (22221818014).

语种：英文

外文关键词：Escherichia coli; metabolic engineering; transcriptional regulator; sulfur conversion rate; l-cysteine; agricultural/food industries

摘要：Cysteine is a commercially important sulfur-containing amino acid widely used as a supplement in the agricultural and food industries. It is extremely desirable to achieve a high sulfur conversion rate in the fermentation-based cysteine production. Here, the metabolic engineering of Escherichia coli was performed to enhance the sulfur conversion rate in cysteine biosynthesis. Accordingly, the reduction of sulfur loss by the regulator decR and its yhaOM operons were deleted. serACB was integrated into chromosome with constitutive promoter to coordinately increase sulfur utilization. The sulfur assimilation pathways and sulfur transcriptional regulator cysB were overexpressed to regulate sulfur metabolism and enhance sulfur conversion significantly. After the process optimization in fed-batch fermentation, LH16 [SLH02 Delta yhaM Ptrc1-serACB-cysM-nrdH-(pLH03, pTrc99a-cysB)] produced 7.5 g/L of cysteine with a sulfur conversion rate of 90.11%. These results indicate that cysteine production by LH16 is a valuable process in the agricultural and food industries.