文献类型：期刊文献

英文题名：The characteristics of open-tubular capillary electrochromatography columns with series/mixed stationary phases constructed with magnetic nanoparticle coating

作者：Zhu, Yaxian[1];Zhang, Lingyi[1];Qian, Junhong[1];Zhang, Weibing[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2013

卷号：104

起止页码：173

外文期刊名：TALANTA

收录：;EI(收录号：20130215878402);WOS:【SCI-EXPANDED(收录号:WOS:000319310900027)】；

基金：We gratefully acknowledge the support of the Specialized Research Fund for the Doctoral Program of Higher Education of China (20100074110016) and the National Natural Science Foundation of China (21075039, 21105027).

语种：英文

外文关键词：CEC; Open-tubular column; Series stationary phases; Mixed stationary phases; Magnetic nanoparticle

摘要：Novel open-tubular capillary electrochromatography (OT-CEC) systems with core/shell magnetic nanoparticles modified by amino or C-18 groups as stationary phase were constructed by immobilizing nanoparticles in the capillary with permanent magnets. Influence of preparation method of OT-CEC column with series stationary phases (continuous two-dimension) on column performance and effect of dispersant on capability of OT-CEC column prepared by stationary phases with mixed functionalities (mixed stationary phases) were investigated in details to achieve stable preparation. Organic acids were used to evaluate the OT-CEC systems, and the relative column efficiency of salicylic acid was 420,000 plates/m for series stationary phases, while that of benzoic acid reached 480,000 plates/m for mixed stationary phases. The excellent within-column and between-column repeatability (n=5) testified with the RSDs of retention time were less than 0.44% and 10.20% for series stationary phases and 1.65% and 4.29% for mixed stationary phases. The two OT-CEC systems were further applied to separation of the aqueous extract of Rhizoma gastrodiae. Comparing with normal OT capillary column, the new systems show extra high column efficiency due to large surface areas of nanoparticles and multiple separation mechanisms, and they have great potential in the method development for the analysis of complicated samples. (C) 2012 Elsevier B.V. All rights reserved.