文献类型：期刊文献

英文题名：Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes

作者：He, Xiao-Peng[1];Zhu, Bi-Wen[1];Zang, Yi;Li, Jia[2];Chen, Guo-Rong[1,2];Tian, He[1];Long, Yi-Tao[1]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat & Inst Fine Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China

年份：2015

卷号：6

期号：3

起止页码：1996

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20150800561642);WOS:【SCI-EXPANDED(收录号:WOS:000349832600050)】；

基金：We thank the 973 project (2013CB733700), the Science Fund for Creative Research Groups (21421004), the National Science Fund for Distinguished Young Scholars (21125522, 81125023), the National Natural Science Foundation of China (21202045), the Key Project of Shanghai Science and Technology Commission (13NM1400900), the Program of Shanghai Subject Chief Scientist (13XD1404300) and the Fundamental Research Funds for Central Universities. Ms Huan Wang is thanked for her contribution to the cytotoxicity experiments.

语种：英文

外文关键词：Diagnosis - Graphite electrodes

摘要：Expression of specific transmembrane receptors by cells frequently represents an important signature of diseases, but this dynamic event can hardly be monitored directly with live cells due to the limitation of current biochemical techniques. Here we develop a pyrenyl glycoanthraquinone construct that can be firmly immobilized on a graphene-spotted screen printed electrode via strong p-interactions. The inherent current signal produced by the surface-confined glycoquinone can be used to detect selective sugar-protein recognitions with simple electrochemical techniques and portable facilities. Importantly, we demonstrate that the level of pathogenic receptors expressed by different types of live cells can be tracked with the electrode system in a label-free manner, providing a useful tool for the on-demand disease diagnosis as well as basic biochemical studies.