文献类型：期刊文献

英文题名：Theoretical investigation on the performance of DNA electrophoresis under programmed step electric field strength: Two-step condition

作者：Ni, Yi[1];Liu, Chenchen[2];Chen, Qinmiao[1];Zhu, Xifang[3];Dou, Xiaoming[1,3]

机构：[1]E China Univ Sci & Technol, Grad Sch Sci, Inst Photon & Biomed, Shanghai 200237, Peoples R China;[2]Shanghai Univ Sci & Technol, Engn Res Ctr Opt Instrument & Syst, Shanghai Key Lab Modern Opt Syst, Minist Educ, Shanghai 201800, Peoples R China;[3]Changzhou Inst Technol, Sch Optoelect Engn, Changzhou, Jiangsu, Peoples R China

年份：2015

卷号：38

期号：20

起止页码：3638

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20153901299984);WOS:【SCI-EXPANDED(收录号:WOS:000363414300020)】；

基金：This research was supported by the National Natural Science Foundation of China (No. 21305089), Innovation Program of Shanghai Municipal Education Commission (No. 13YZ073). We acknowledged on partly financial support by East China University of Science and Technology (No. YK0142119).

语种：英文

外文关键词：Capillary electrophoresis; DNA; Programmed step electric field strength; Two-step electric field strength

摘要：Programmed step electric field strength is a simple-to-use technique that has already been reported to be effective to enhance the efficiency or speed of DNA electrophoresis. However, a global understanding and the details of this technique are still vague. In this paper, we investigated the influence of programmed step electric field strength by theoretical calculation and concentrated on a basic format named as two-step electric field strength. Both subtypes of two-step electric field strength conditions were considered. The important parameters, such as peak spacing, peak width, resolution, and migration time, were calculated in theory to understand the performance of DNA electrophoresis under programmed step electric field strength. The influence of two-step electric field strength on DNA electrophoresis was clearly revealed on a diagram of resolution versus migration time. Both resolution and speed of DNA electrophoresis under two-step electric field strength conditions are simply expressed by the shape of curves in the diagram. The possible shapes of curve were explored by calculation and shown in this paper. The subtype II of two-step electric field strength brings drastic variation on the resolution. Its limitations of enhancement and deterioration of resolution were predicted in theory.