文献类型：期刊文献

中文题名：双孔块状SiO_2载体材料的孔结构及其酶活性的研究

英文题名：Double-pore Structure and Enzyme Activity of SiO_2 Monolithic Carrier

作者：杨洪斌[1];陈奇[1];宋鹂[1];陆剑英[1];李会平[1]

机构：[1]华东理工大学材料科学与工程学院,上海200237

年份：2007

卷号：23

期号：1

起止页码：164

中文期刊名：无机化学学报

外文期刊名：Chinese Journal of Inorganic Chemistry

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市科委科技攻关项目(No.0252NM006)资助

语种：中文

中文关键词：双孔结构;多孔块体;溶胶-凝胶法;酶载体;酶活性

外文关键词：double-pore structure; porous monolithic; sol-gel; enzyme carrier; enzyme activity

摘要：SiO2 monoliths with double-pore structure were synthesized with tetraethyl orthosilicate, polyethylene glycol, starch, ethanol and ammonia as the main raw materials via a sol-gel route. The effect of different content of starch of the samples was studied on the mesopore structure, macropore appearance, hydrothermal stability and enzyme activity to immobilize gluczyme. The results indicate that porous blocks with mesopores of 10~12 nm and macropores of 10~30 μm can be formed by the removal of polyethylene glycol and starch after heating at 600 ℃. The minimal density of the obtained samples was 0.34 g·cm-3 and the maximal porosity was 76%. After being immersed in water at 80 ℃ for 7 d, the density, porosity and shape of N2 adsorption-desorption curves of the samples were almost unchanged, showing that the sample has a good hydrothermal stability. The initial enzyme activity of sample P2KD30 was 11 190 U, and definite enzyme activity could be maintained after five cycles.
SiO2 monoliths with double-pore structure were synthesized with tetraethyl orthosilicate, polyethylene glycol, starch, ethanol and ammonia as the main raw materials via a sol-gel route. The effect of different content of starch of the samples was studied on the mesopore structure, macropore appearance, hydrothermal stability and enzyme activity to immobilize gluczyme. The results indicate that porous blocks with mesopores of 10-12 nm and macropores of 10-30 I^m can be formed by the removal of polyethylene glycol and starch after heating at 600℃. The minimal density of the obtained samples was 0.34 g·cm^-3 and the maximal porosity was 76%. After being immersed in water at 80 ℃ for 7 d, the density, porosity and shape of N2 adsorption-desorption curves of the samples were almost unchanged, showing that the sample has a good hydrothermal stability. The initial enzyme activity of sample P2KD30 was 11 190 U, and definite enzyme activity could be maintained after five cycles.