文献类型：期刊文献

英文题名：Preparation and Characterization of Thermosensitive and Folate Functionalized Pluronic Micelles

作者：Yang, Cheng[1,2];Zhao, Hongli[1,2];Yuan, Huihui[1,2];Yu, Ronghua[1,2];Lan, Minbo[1,2,3]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2013

卷号：13

期号：10

起止页码：6553

外文期刊名：JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY

收录：;EI(收录号：20135017069499);WOS:【SCI-EXPANDED(收录号:WOS:000328704000011)】；

基金：This work was supported by the National Natural Science Foundation of China (NSFC, contract No. 21074033) and the Science and Technology Commission of Shanghai Municipality (STCSM, contract No. 11nm0505700).

语种：英文

外文关键词：Copolymer Micelles; Folate Functionalized; Thermosensitive; Tumor-Targeted

摘要：In this study, thermosensitive and folate functionalized poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide)-ploy(N-isopropylacrylamide-co-hydroxyethyl methacrylate) (FA-Pluronic-PNH) copolymer was synthesized. The structure and molecular weight of the copolymer were confirmed by H-1 NMR, FT-IR and GPC, respectively. The lower critical solution temperature (LCST) of the copolymer was 39.8 degrees C. By employing doxorubicin (DOX) as a model drug, folate receptor-targeted DOX-loaded micelles were further formed on the copolymer. The blank and DOX-loaded micelles both exhibited nearly spherical shapes and their average diameters were 35 nm and 50 nm, respectively. The in vitro release behaviors of the DOX-loaded micelles were temperature-dependent and the release rate of DOX at 42 degrees C (above LCST) was faster than that at 37 degrees C (below LCST). Furthermore, the cytotoxicity assays of free DOX and DOX-loaded micelles on human cervical cancer cell lines HeLa and human lung cancer cell lines A549 demonstrated that folate increased the cellular uptake of the micelles within targeted cells that vastly over-expressed folate receptors.