文献类型：期刊文献

英文题名：Application of oxygen uptake rate and response surface methodology for erythromycin production by Saccharopolyspora erythraea

作者：Zou, Xiang[1,2];Hang, Hai-feng[1];Chen, Chang-fa[1];Chu, Ju[1];Zhuang, Ying-ping[1];Zhang, Si-liang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southwest Univ, Sch Pharmaceut Sci, Chongqing 400715, Peoples R China

年份：2008

卷号：35

期号：12

起止页码：1637

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000260837900009)】；

基金：This work was financially supported by the National High Technology Research and Development Program of China (863 Program), No. 2006AA020304 and the Major State Basic Research Development Program of China (973 Program), No. 2007CB714303.

语种：英文

外文关键词：Bioreactors; Erythromycin; Fed-batch culture; Physiology; Process integration; Response surface methodology

摘要：A process for efficient production of erythromycin by Saccharopolyspora erythraea using statistical designs and feeding strategy was developed. The critical nutrient components were selected in accordance with fractional factorial design and were further optimized via response surface methodology. Three significant components (ZnSO(4), citric acid threonine) were identified for the optimization study. The optimum levels of these significant variables were determined with Box-Behnken design, which were ZnSO(4) 0.039 g/l, citric acid 0.24 g/l and threonine 0.42 g/l, respectively. A novel feeding strategy based on oxygen uptake rate (OUR) measurement was developed successfully to increase the flux of erythromycin biosynthesis, in which the optimized nutrient components was fed in the 50 l stirred bioreactor when OUR began to decline at 46 h. The maximum erythromycin production reached 10,622 U/ml, which was 11.7% higher than the control in the same cultivation conditions. It was the first report to integrate physiological parameter OUR and statistical methods to optimize erythromycin production.