文献类型：期刊文献

中文题名：固定化L-天冬酰胺酶间歇催化反应的动力学模型

英文题名：Studies on Kinetics Model of Immobilized Lasparaginase in Batch Reaction System

作者：周纪宁[1];金浩[1];江体乾[1]

机构：[1]华东理工大学化工学院

年份：1999

卷号：25

期号：3

起止页码：269

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：L-天冬酰胺酶;固定化酶;动力学;催化反应

外文关键词：Lasparaginase; chitosan; immobilized enzyme; reaction kinetics

摘要：测定了３７°Ｃ下以固定化Ｌ－天冬酰胺酶为催化剂，５０μｍｏｌ／ＬＬ－天冬酰胺的Ｔｒｉｓ缓冲液（ｐＨ＝７．４）为底物的时歇反应转化率，并用建立的数学模型对反应过程进行拟合。计算结果表明，提出的传质－催化反应动力学模型能较好地描述反应过程，并揭示固定化酶的传质－催化过程受扩散作用控制。
Immobilized Lasparaginase is selected as the studied object. The conversion rate￣elapsed time curve is obtained at 37C in batch and catalytic reaction, with 50mol/L Lasparagine in Tris buffer solution (pH=7.4) as the reaction substrate. The mathematical model is used to simulate the whole reaction process. The calculation results from the models demonstrate that the suggested mass transfercatalysis reaction kinetics models are in good agreement with the actual situation and the whole processes are controlled by diffusion effects.