文献类型：期刊文献

英文题名：Biodegradation of phenanthrene by Rhizobium petrolearium SL-1

作者：Huang, X.[1,2];Shi, J.[3];Cui, C.[3];Yin, H.[4];Zhang, R.[1,2];Ma, X.[1];Zhang, X.[1]

机构：[1]Chinese Acad Agr Sci, Key Lab Microbial Resources Collect & Preservat, Minist Agr, Inst Agr Resources & Reg Planning, Beijing, Peoples R China;[2]Capital Normal Univ, Coll Resources Environm & Tourism, Beijing, Peoples R China;[3]East China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Sch Resources & Environm Engn, Shanghai, Peoples R China;[4]Cent S Univ, Sch Minerals Proc & Bioengn, Changsha, Hunan, Peoples R China

年份：2016

卷号：121

期号：6

起止页码：1616

外文期刊名：JOURNAL OF APPLIED MICROBIOLOGY

收录：;EI(收录号：20231714002723);WOS:【SCI-EXPANDED(收录号:WOS:000392840500012)】；

基金：This work was supported by the National High Technology Research and Development Program of China (2012AA101403), National Natural Science Foundation of China (41271273 and 41301329), and the Key '948' Project of Ministry of Agriculture of China (2011-G25).

语种：英文

外文关键词：biodegradation; degrading properties; metabolic pathway; phenanthrene; Rhizobium

摘要：Aims: We aimed to investigate the phenanthrene degradation characteristics and possible phenanthrene degradation pathways of Rhizobium petrolearium SL-1. Methods and Results: Using high-performance liquid chromatography (HPLC), UV-visible and gas chromatography-mass spectrometry (GC-MS) analysis, the phenanthrene-degrading properties and metabolites of Rh. petrolearium SL-1 were analysed, and then combined with genome-based analysis to elucidate the possible biodegradation pathway of phenanthrene in Rh. petrolearium SL-1. The results of the analyses showed that phenanthrene (100 mg l(-1)) was completely degraded by strain SL-1 at 35 degrees C, 0.02% salinity and pH 9.0 within 3 days. A wide range of polycyclic aromatic hydrocarbons, including naphthalene, fluorene, anthracene and pyrene, could also be degraded by this strain. Conclusions: Based on the identified metabolites, the utilization of probable intermediates and the presence of putative phenanthrene catabolic genes, we concluded that phenanthrene was degraded via two different routes, namely the 'naphthalene' and the 'phthalic acid' routes. Significance and Impact of the Study: For the first time, this study shows the degradation pathway of phenanthrene by a Rhizobium strain. Because of its excellent stress resistance, metabolic versatility, high degradation efficiency and potential application in phytoremediation, Rh. petrolearium SL-1 is a potential candidate for the bioremediation of polycyclic aromatic hydrocarbons-contaminated areas.