文献类型：期刊文献

中文题名：麦芽根中5′-磷酸二酯酶的制备及性质

英文题名：Preparation and Properties of 5′-Phosphodiesterase from Barley Rootlet

作者：李德莹[1];邱蔚然[1];丁庆豹[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2002

卷号：28

期号：4

起止页码：376

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2002447167718);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：制备;麦芽根;5′-磷酸二酯酶;5′-核苷酸;核糖核酸;热稳定性;pH稳定性;啤酒废料

外文关键词：barley rootlet; 5′ phosphodiesterase; 5′ nucleotide; RNA

摘要：从麦芽根中提取 5′-磷酸二酯酶 ,酶活可达 474U/ m L。将所得酶液用于水解酵母核糖核酸 ,得到 5′-CMP、UMP、GMP、AMP 4种单核苷酸。最佳反应条件为 :底物 RNA浓度为0 .0 1 g/ m L,每毫升底物的酶用量为 3 0 U,酶解温度 70°C,p H7.0 ,时间 2 h,5′-核苷酸转化率达80 %。
Extraction of 5′ phosphodiesterase from barley root was conducted. The enzyme activity obtained was 474 U/mL. When ribonucleic acids from yeast were digested with the enzyme solution, 4 kinds of nucleotides, 5′ CMP, UMP, GMP and AMP were resulted. The optimum conditions for the reactions were as follows: concentration of substrate RNA, 0.01g/mL; enzyme activity used for per mL of substrate, 30U; digestion temperature, 70°C; pH7.0; reaction time, 2h. The yield of 5′ nucleotide attained 80%.