文献类型：期刊文献

英文题名：An Amperometric Biosensor Based on Glucose Oxidase Immobilized in a Sol-Gel Polyvinyl Alcohol/Silica Hybrid Composite Film on a Prussian Blue Modified Electrode

作者：Zuo, Shao-Hua[2];Zhao, Hong-Li[2];Zhang, Ling-Fan[2];Yuan, Hui-Hui[2];Lan, Min-Bo[1,2,3];Lawrence, Geoffrey A.[4];Wei, Gang[5]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Chinese Educ Minist, Shanghai 200237, Peoples R China;[4]Univ Newcastle, Sch Environm & Life Sci, Discipline Chem, Callaghan, NSW 2308, Australia;[5]CSIRO Ind Phys, Sydney, NSW 2070, Australia

年份：2010

卷号：3

期号：4

起止页码：476

外文期刊名：ADVANCED SCIENCE LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000282013500023)】；

基金：The support of the Science and Technology Commission of Shanghai Municipality (STCSM; No. 09391911500) is gratefully acknowledged.

语种：英文

摘要：An amperometric glucose biosensor was fabricated by entrapment of glucose oxidase in a composite matrix of silica sol-gel and polyvinyl alcohol physically layered on top of a Prussian blue-modified Pt electrode. This biosensor functions through the detection of hydrogen peroxide produced by the glucose oxidase-catalyzed oxidation of glucose at -0.05 V. The biosensor exhibited high selectivity, and a sensitivity of 3.41 mu A/mM, due to a combination of electroreduction of H2O2 at the surface of the modified Pt electrode at low potential and a sympathetic microenvironment for enzyme immobilization provided by the silica sol-gel. The biosensor had a linear response to glucose over the range 5.0 x 10(-5) to 2.5 x 10(-3) M, with a response time of less than 8 sec. The detection limit of the biosensor is 4.0 mu M, based on a signal-to-noise ratio of 3. The biosensor exhibits satisfactory long-term stability; 85% of its original activity was retained after 30 days of storage in phosphate buffer (pH 6.9; 0.1 M) at 4 degrees C.