文献类型：期刊文献

英文题名：Methanogenic biodegradation of C13 and C14 n-alkanes activated by addition to fumarate

作者：Ji, Jia-Heng[1,2];Liu, Yi-Fan[1,2];Zhou, Lei[1,2];Irfan, Muhammad[1,2,3];Mbadinga, Serge Maurice[1,2];Pan, Pan[1,2];Chen, Jing[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Sand, Wolfgang[4,5,6];Gu, Ji-Dong[7];Mu, Bo-Zhong[1,2,8]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Univ Engn & Technol, Dept Chem Polymer & Composite Mat Engn, KSK Campus, Lahore 54890, Pakistan;[4]Donghua Univ, Coll Environm Sci & Engn, Text Pollut Controlling Engn Ctr, Minist Environm Protect, Shanghai 201620, Peoples R China;[5]Freiberg Univ Min & Technol, Inst Biosci, D-09599 Freiberg, Germany;[6]Univ Duisburg Essen, Biofilm Ctr, Essen, Germany;[7]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China;[8]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：153

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20202408811371);WOS:【SCI-EXPANDED(收录号:WOS:000564599100007)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41530318), NSFC/RGC Joint Research Fund (No. 41161160560), Fundamental Research Funds for the Central Universities (Nos. 222201817017, 50321101917017, 22221818014), and Research Program of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：1-methylalkyl succinate; Alkane; Fumarate addition; Methanogenesis; Smithella

摘要：n-Alkanes comprise a substantial proportion of crude oil; however, their degradation mechanism under methanogenic conditions is still unclear. Here, we investigated the initial activation mechanism of biodegradation of n-tridecane and n-tetradecane by methanogenic cultures enriched from a low-temperature oilfield production water. Methane accumulated in alkane-degrading enrichment cultures during the whole incubation period of 364 days, demonstrating that alkanes were actively degraded. Putative metabolites consistent with succinylated n-tridecane and n-tetradecane were detected, together with alkylsuccinate synthase-like genes, suggesting that alkanes were initially activated upon the addition of fumarate. Smithella and Desulfatibacillum in conjunction with hydrogenotrophic and acetoclastic methanogens were proposed to be important alkane degraders under methanogenic conditions. The results of this work added evidence that fumarate addition is a key activation mechanism in methanogenic alkane degradation.