文献类型：期刊文献

英文题名：Efficient reduction of aromatic ketones with NADPH regeneration by using crude enzyme from Rhodotorula cells and mannitol as cosubstrate

作者：Yang, Wei[1];Xu, Jian-He[1];Pan, Jiang[1];Xu, Yi[1];Wang, Zhi-Long[2]

机构：[1]E China Univ Sci & Technol, Lab Biocatalysis & Bioproc, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China

年份：2008

卷号：42

期号：1

起止页码：1

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000259712700001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 20576037), Ministry of Education (No. 20050251011), and Ministry of Science and Technology, P.R. China (Nos. 2006AA02Z205 & 2007AA02Z225).

语种：英文

外文关键词：biocatalysis; brotransformations; chromatography; optimization; NADPH regeneration; Rhodotorula sp AS2.2241 ketoreductase

摘要：An enzyme-coupled system, containing a ketoreductase for prochiral ketones reduction and a mannitol dehydrogenase for NADPH regeneration, was discovered from the cell-free extract of Rhodotorula sp. AS2.2241 and used Successfully for the asymmetric reduction of 2-acetylpyridine, giving (S)-1-(2pyridine)ethanol in >99% ee With recycling of NADPH for 4220 times. Moreover, the endogenously coupled enzymes system could also reduce Various aromatic ketones Such as 3- and 4-acetylpyridines with 96-99% ee. (C) 2008 published by Elsevier B.V.