文献类型：期刊文献

英文题名：Enhancing Biosynthesis of a Ginsenoside Precursor by Self-Assembly of Two Key Enzymes in Pichia pastoris

作者：Zhao, Chengcheng[1];Gao, Xin[1];Liu, Xinbin[1];Wang, Yong[2];Yang, Shengli[1];Wang, Fengqing[1];Ren, Yuhong[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China

年份：2016

卷号：64

期号：17

起止页码：3380

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20162102414782);WOS:【SCI-EXPANDED(收录号:WOS:000375521300010)】；

基金：This work was funded by the National Special Fund for the State Key Laboratory of Bioreactor Engineering (2060204) and the National Basic Research Program of China (973, Program No. 2012CB721003, No. 2012CB721103).

语种：英文

外文关键词：ginsenosides; self-assembly; Pichia pastoris; dammarenediol-II

摘要：Ginsenosides from the edible and medicinal plant ginseng have demonstrated various pharmacological activities. However, producing ginsenoside efficiently remains a challenge. Engineering metabolic pathways through protein assembly in yeast is a promising way for ginsenoside production. In the biosynthetic pathway of ginsenosides, dammarenediol-II synthase and squalene epoxidase are two key enzymes that determine the production rate of the dammarane-type ginsenoside precursor dammarenediol-II. In this work, a strategy to enhance the biosynthesis of dammarenediol-II in Pichia pastoris was developed by the self-assembly of the two key enzymes via protein protein interaction. After being modified by interacting proteins, the two enzymes were successfully co-localized, resulting in a 2.1-fold enhancement in dammarenediol-II yields.