文献类型：期刊文献

英文题名：Fabrication and preliminary study of a biomimetic tri-layer tubular graft based on fibers and fiber yarns for vascular tissue engineering

作者：Wu, Tong[1];Zhang, Jialing[2];Wang, Yuanfei[3];Li, Dandan[4];Sun, Binbin[1];El-Hamshary, Hany[5,6];Yin, Meng[2];Mo, Xiumei[1]

机构：[1]Donghua Univ, State Key Lab Modificat Chem Fibers & Polymer Mat, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Childrens Med Ctr, Sch Med, Dept Cardiothorac Surg, Shanghai 200127, Peoples R China;[3]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Donghua Univ, Coll Mat Sci & Engn, Shanghai 201620, Peoples R China;[5]King Saud Univ, Coll Sci, Dept Chem, Riyadh 11451, Saudi Arabia;[6]Tanta Univ, Fac Sci, Dept Chem, Tanta 31527, Egypt

年份：2018

卷号：82

起止页码：121

外文期刊名：MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录：;EI(收录号：20173504086195);WOS:【SCI-EXPANDED(收录号:WOS:000414886400014)】；

基金：This research was supported by the National Major Research Program of China (2016YFC1100202), National Natural Science Foundation of China (31470941, 81671833), Science and Technology Commission of Shanghai Municipality (15JC1490100, 15441905100), Fundamental Research Funds for the Central Universities (CUSF-DH-D2017047), light of textile project (J201404), Pudong New Area Science and Technology Development Fund Minsheng Scientific Research (Medical and Health) Project (PKJ2016-Y33), Collaborative Innovation Center for Translational Medicine (TM201504) and Ai You Foundation (2017SCMC-AY002). The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding through the research group project (No. RGP-201).

语种：英文

外文关键词：Tri-layer tubular graft; Fibers; Fiber yarns; Vascular tissue engineering

摘要：Designing a biomimetic and functional tissue-engineered vascular graft has been urgently needed for repairing and regenerating defected vascular tissues. Utilizing a multi-layered vascular scaffold is commonly considered an effective way, because multi-layered scaffolds can easily simulate the structure and function of natural blood vessels. Herein, we developed a novel tri-layer tubular graft consisted of Poly(L-lactide-co-caprolactone)/collagen (PLCL/COL) fibers and Poly(lactide-co-glycolide)/silk fibroin (PLGA/SF) yarns via a three-step electrospinning method. The tri-layer vascular graft consisted of PLCL/COL aligned fibers in inner layer, PLGA/SF yarns in middle layer, and PLCL/COL random fibers in outer layer. Each layer possessed tensile mechanical strength and elongation, and the entire tubular structure provided tensile and compressive supports. Furthermore, the human umbilical vein endothelial cells (HUVECs) and smooth muscle cells (SMCs) proliferated well on the materials. Fluorescence staining images demonstrated that the axially aligned PLCL/COL fibers prearranged endothelium morphology in lumen and the circumferential oriented PLGA/SF yarns regulated SMCs organization along the single yarns. The outside PLCL/COL random fibers performed as the fixed layer to hold the entire tubular structure. The in vivo results showed that the tri-layer vascular graft supported cell infiltration, scaffold biodegradation and abundant collagen production after subcutaneous implantation for 10 weeks, revealing the optimal biocompatibility and tissue regenerative capability of the tri-layer graft. Therefore, the specially designed tri-layer vascular graft will be beneficial to vascular reconstruction.