文献类型：期刊文献

英文题名：Combination of normal-phase medium-pressure liquid chromatography and high-performance counter-current chromatography for preparation of ginsenoside-Ro from panax ginseng with high recovery and efficiency

作者：Cheng, Yijun[1,2];Liang, Qionglin[1];Hu, Ping[3];Wang, Yiming[1];Jun, Frank Wu[4];Luo, Guoan[1,2]

机构：[1]Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr Tradit Chinese Med, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]XiangCam TCM Res Ctr Ltd, Unit 16, Cambridge CB22 3AT, England

年份：2010

卷号：73

期号：3

起止页码：397

外文期刊名：SEPARATION AND PURIFICATION TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000280047200008)】；

基金：This research was funded by National Natural Science Foundations of China (30860381, 20805026, 90917003) and the National Basic Research Program (973 Program) of China (2007CB714505) and key technology program (2006BAI08B04-01). Also the authors would like to thank colleagues at Tsinghua University-Miss Li Chen; at East China University of Science and Technology-Dr. Min Zhang for their help and support during experiment work.

语种：英文

外文关键词：Ginsenoside-Ro; High-performance counter-current; chromatography; Normal-phase medium-pressure liquid; chromatography; Oleanane-type saponins; Panax ginseng

摘要：Ginsenoside-Ro which belongs to the oleanane-type saponins has anti-inflammatory, anti-platelet, anticomplementary and immunomodulatory activities. The present paper described the preparation of ginsenoside-Ro from panax ginseng with high recovery and efficiency by combination of normal-phase medium-pressure liquid chromatography (NP-mPLC) and high-performance counter-current chromatography (HPCCC). The crude sample was preliminarily chromatographed by NP-MPLC to enrich ginsenoside-Ro to the purity of 70.2% with 95.0% recovery. Then, the enriched sample was further purified by HPCCC with a solvent system composed of ethyl acetate-isopropanol-0.1% formic acid (3:1:5, v/v), where 61 mg ginsenoside-Ro was obtained from 100 mg enriched sample with 96.0% purity and 83.4% recovery. The overall recovery of ginsenoside-Ro was 79.2%, whose structure was identified by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) finally. (C) 2010 Elsevier B.V. All rights reserved.