文献类型：期刊文献

英文题名：De novo transcriptome sequencing of marine-derived Aspergillus glaucus and comparative analysis of metabolic and developmental variations in response to salt stress

作者：Liu, Shaomei[1];Li, Jiaxin[1];Wu, Yuan[1];Ren, Yanna[1];Liu, Qi[1];Wang, Qiyao[1];Zhou, Xiangshan[1];Cai, Menghao[1];Zhang, Yuanxing[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：39

期号：3

起止页码：317

外文期刊名：GENES & GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000395001100008)】；

基金：This work was supported by National Natural Science Funds of China (41306121), the Fundamental Research Funds for the Central Universities (22A201514040) and Grants of young and middle-aged leading science and technology innovation talents from ministry of science and technology of China. We acknowledge Shanghai Personal Biotechnology Co., Ltd. for the sequencing, the kind help of data analysis and manuscript revision.

语种：英文

外文关键词：Marine fungi; Aspergillus glaucus; Salt stress; RNA-Seq; Asexual development; Secondary metabolites

摘要：Aspergillus glaucus HB1-19 is a typical marine-derived fungus preferring the dependence on sea water for its growth, asexual development and polyketides biosynthesis. Therein, salt stress greatly functions even in superior to light illumination, which is also a critical regulation signal for fungi. Here, comparative RNA-seq analysis of this strain was performed under conditions of salt-stress + dark (group A), non salt-stress + dark (group B), salt-stress + light (group C). The RNA-seq generated a total of 19,024 unigenes with an average length of 1415 bp. Differentially expressed genes were very similar between group A and group C but greatly differed between group A and group B, proving that salt stress functioned superior to light illumination globally. Salt stress highly enhanced primary metabolism and activated Ras and MAPK signaling pathways. There seems no direct interaction between asexual development and polyketides biosynthesis. Salt stress inhibited terpenoids biosynthesis but showed little influences on polyketide pathway as well as other secondary metabolism pathways. These findings provide a better understanding of marine fungi adapting to marine environment. Also, it indicates that the so-called 'salt stress-induced' may truly be a 'metal ions-induced' for biosynthesis of secondary metabolites in marine fungi.