文献类型：期刊文献

中文题名：Co^(2+)对红霉素生物合成过程的影响

英文题名：Effect of Co^(2+) on the Erythromycin Biosynthesis

作者：杜雯[1];陈长华[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2006

卷号：32

期号：11

起止页码：1280

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：20070310373198);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：河南省第三批科技发展重点攻关项目(05230511300)

语种：中文

中文关键词：红霉素;糖代谢;甲基丙二酰CoA异构酶;甲基丙二酰CoA羧基转移酶;有机酸

外文关键词：erythromycin; glucose metabolism; methylmalonyl-coenzyme A mutase ; methylmalonylcoenzyme A transcaboxylase; organic acid

摘要：研究了红霉素生物合成过程中糖代谢相关的关键酶:己糖激酶、6-磷酸葡萄糖脱氢酶、磷酸果糖激酶和异柠檬酸脱氢酶,以及合成红霉内酯环前体甲基丙二酰CoA相关的酶:甲基丙二酰CoA异构酶和甲基丙二酰CoA羧基转移酶的时序变化,并测定了过程中有机酸的含量变化。实验表明:Co2+能大幅提高红霉素效价。这可能与Co2+能明显提高甲基丙二酰CoA异构酶与甲基丙二酰CoA羧基转移酶的比活,并在一定程度上促进糖代谢有关。
Erythromycin biosynthesis is a highly complicated process, which involves both primary metabolism and secondary metabolism. The activities of the key enzymes related to glucose metabolism such as glucose kinase, glucose-6-phosphate dehydrogenase, phosphofrutokinase and isocitrate dehydrogenase in Saccharopolyspora erythraea, and the activities of the enzymes involved in secondary metabolism, such as methylmalonyl-coenzyme A mutase, and methylmalonyl-coenzyme A transcaboxylase, were determined. Some organic acids in fermentation broth were also analyzed. Results show that Co^2＋ increases erythromyein biosynthesis. It maybe owe to improvement of the specific activities of methylmalonyl-coenzyme A mutase and methylmalonyl-coenzyme A transcaboxylase by Co^2＋. Meanwhile, it also increases the flux of glucose metabolism pathway.