文献类型：期刊文献

英文题名：Metabolic engineering of Corynebacterium glutamicum S9114 to enhance the production of L-ornithine driven by glucose and xylose

作者：Zhang, Bin[1];Gao, Ge[1];Chu, Xiao-He[2];Ye, Bang-Ce[1,2]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Hangzhou 310014, Zhejiang, Peoples R China

年份：2019

卷号：284

起止页码：204

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20191406722289);WOS:【SCI-EXPANDED(收录号:WOS:000464942500025)】；

基金：We would like to thank National Natural Science Foundation of China [grant numbers, 31730004 and 21575089] for providing foundation.

语种：英文

外文关键词：Corynebacterium glutamicum; L-ornithine; Metabolic engineering; Glucose; Xylose

摘要：L-ornithine, an important amino acid, is widely used in food and medicine industries. L-ornithine production mainly relies on microbial fermentation, which may not meet the industrial requirement owing to the poor fermentation ability of available strains. Herein, mscCG2 deletion, CgS9114 12202 (gdh2) overexpression and rational modulation in tricarboxylic acid cycle was firstly demonstrated to increase L-ornithine production in engineered Corynebacterium glutamicum S9114. By further modulate glucose utility result in strain SO26 that produced 38.5 g/L or 43.6 g/L of L-ornithine in shake flask and fed-batch fermentation, respectively. This was 25% higher than that of the original strain (30.8 g/L) and exhibits highest titer reported in shake-flask. Moreover, the incorporation of xylose pathway in the engineered strain resulted in the highest L-ornithine production titer (18.9 g/L) and yield (0.40 g/g xylose) with xylose substrate. These results illustrate the tremendous potential of the engineered strain C. glutamicum S9114 in L-ornithine production.