文献类型：期刊文献

英文题名：L-Cysteine Production in Escherichia coli Based on Rational Metabolic Engineering and Modular Strategy

作者：Liu, Han[1];Fang, Guochen[1];Wu, Hui[1,2,3];Li, Zhimin[1];Ye, Qin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]China Natl Light Ind Council, Key Lab Biobased Mat Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：13

期号：5

外文期刊名：BIOTECHNOLOGY JOURNAL

收录：;EI(收录号：20180904837433);WOS:【SCI-EXPANDED(收录号:WOS:000431621000015)】；

基金：This study was supported by Shanghai Committee of Science and Technology (Grant No. 13DZ1930202) and the National Science Foundation for Young Scientist of China (Grant No. 21406065).

语种：英文

外文关键词：Escherichia coli; L-cysteine; modular strategy; rational metabolic engineering

摘要：L-cysteine is an amino acid with important physiological functions and has a wide range of applications in medicine, food, animal feed, and cosmetics industry. In this study, the L-cysteine synthesis in Escherichia coliEscherichia coli is divided into four modules: the transport module, sulfur module, precursor module, and degradation module. The engineered strain LH03 (overexpression of the feedback-insensitive cysE and the exporter ydeD in JM109) accumulated 45.8mgL(-1) of L-cysteine in 48hr with yield of 0.4% g/g glucose. Further modifications of strains and culture conditions which based on the rational metabolic engineering and modular strategy improved the L-cysteine biosynthesis significantly. The engineered strain LH06 (with additional overexpression of serA, serC, and serB and double mutant of tnaA and sdaA in LH03) produced 620.9mgL(-1) of L-cysteine with yield of 6.0% g/g glucose, which increased the production by 12 times and the yield by 14 times more than those of LH03 in the original condition. In fed-batch fermentation performed in a 5-L reactor, the concentration of L-cysteine achieved 5.1gL(-1) in 32hr. This work demonstrates that the combination of rational metabolic engineering and module strategy is a promising approach for increasing the L-cysteine production in E. coli.