文献类型：期刊文献

英文题名：Simultaneous determination of sixteen metabolites related to neural tube defects in maternal serum by liquid chromatography coupling with electrospray tandem mass spectrometry

作者：Liang, Xiao-Ping[2];Liang, Qiong-Lin[1];Xia, Jian-Fei[2];Wang, Yong[2];Hu, Ping[3];Wang, Yi-Ming[1];Zheng, Xiao-Ying[4];Zhang, Ting[5];Luo, Guo-An[1,2]

机构：[1]Tsinghua Univ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ, Dept Chem, Beijing, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharmaceut, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Coll Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]Peking Univ, WHO Collaborating Ctr Res Reprod Hlth & Populat S, Inst Populat Res, Beijing, Peoples R China;[5]Capital Inst Pediat, Beijing, Peoples R China

年份：2009

卷号：78

期号：4-5

起止页码：1246

外文期刊名：TALANTA

收录：;EI(收录号：20091512030946);WOS:【SCI-EXPANDED(收录号:WOS:000266055400005)】；

基金：This study was supported by the National Basic Research Program (973 Program) of China (No. 2007CB511903) and the National Natural Science Foundations of China (No. 20805026). We thank the staff and all participants who joined in and contributed to the laborious fieldwork and also appreciate to the volunteer women for their participation in this study.

语种：英文

外文关键词：LC/MS/MS; Folate; Homocysteine; Glutathione; Neural tube defects

摘要：Disturbances in maternal folate. homocysteine. and glutathione metabolism have been reported to be associated with neural tube defects (NTDs). However, the role played by specific components in the metabolic pathways leading to NTDs remains unclear. Thus an analytical method for simultaneous measurement of sixteen compounds involved in such three metabolic pathways by high performance liquid chromatography-tandem mass spectrometry was developed. The use of hydrophilic chromatography column improved the separation of polar analytes and the detection mode of multiple-reaction monitoring (MRM) enhanced the specificity and sensitivity so as to achieve simultaneous determination of three class of metabolites which have much variance in polarity and contents. The influence of parameters such as temperature, pH, flow rate on the performance of the analytes were studied to get an optimal condition. The method was validated for its linearity, accuracy, and precision, and also used for the analysis of serum samples of NTDs-affected pregnancies and normal women. The result showed that the present method is sensitive and reliable for simultaneous determination of as many as sixteen interesting metabolites which may provide a new means to study the underlying mechanism of NTDs as well as to discover new potential biomarkers. (C) 2009 Elsevier B.V. All rights reserved.