文献类型：期刊文献

中文题名：二硫化钼复合材料的构建与对Aβ的灵敏检测

英文题名：Development of MoS2 Composites and Sensitive Detection of Amyloid β-Protein

作者：程雅文[1];豆伟涛[1];陈国荣[1]

机构：[1]华东理工大学结构可控先进功能材料及其制备教育部重点实验室精细化工研究所费林加诺贝尔奖科学家联合研究中心

年份：2019

卷号：45

期号：5

起止页码：759

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学优秀青年基金(21722801)

语种：中文

中文关键词：复合材料;二硫化钼;淀粉样蛋白;灵敏检测

外文关键词：composites;molybdenum disulfide;amyloid β-protein;sensitive detection

摘要：为解决传统检测阿兹海默症标志物Aβ的荧光方法普遍存在灵敏度低、背景干扰大以及操作过程繁琐的缺点,通过超分子自组装的方法将3种不同波长的荧光探针(白藜芦醇(Res)、硫磺素T(ThT)和4-(二氰基亚甲基)-2-甲基-4H-吡喃衍生物(DCM-OH))与二硫化钼进行复合,通过高分辨质谱和高效液相色谱表征了3种化合物的结构以及纯度,利用扫描电镜和透射电镜研究了复合材料的形貌结构,并对两种不同分子量的肽段片段Aβ(1~40)和Aβ(1~42)进行了检测。结果显示:复合材料结构规整均一,3种体系均能实现对Aβ(1~40)和Aβ(1~42)的灵敏检测;通过合成得到的DCM-OH与MoS2的复合体系粒径分布均一,生物相容度好,对Aβ(1~42)有更低的检测下限(6.8nmol/L),具有潜在应用价值。
Amyloid beta peptide (Aβ) has been considered as the hallmark of Alzheimer ’s disease which is neurodegenerative disease and affects many senior citizens.However,drug for the treatment or control of such a disease is still lacking,thus early detection and intervention are particularly important.So far,fluorescence diagnose technique has been developed as an important mean for the detection of Aβ,but there are still many disadvantages in the traditional fluorescence techniques,such as low sensitivity,large background interference,and complicated operation.To address these issues,we synthesized a series of dicyanomethylene- 4H-pyran (DCM-OH) derivatives which were self-assembled with molybdenum disulfide (MoS2) driven by π-π stacking interaction.The commercial products,resveratrol (Res) and thioflavine T (ThT),were used as the control group.The structures and purities of the three compounds were characterized by high resolution mass spectrometry (HRMS) and high performance liquid chromatography (HPLC).The morphologies and structures of the three composites were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).The characterization results display that the three prepared composites are regular and uniform,and can be used to detect indicators of AD in vitro. Moreover,we tested the applicability of the three composites in the detection of Aβ(1?40) and Aβ(1?42) in solution. The results show that the composites are capable of detecting Aβ(1?40) and Aβ(1?42) sensitively.In addition,the composite DCM-OH/MoS2 was more sensitive for the detection of Aβ(1?42) with a LOD (Limit of Detection) of 6.8 nmol/L,which is 9.8 nmol/L for Aβ(1?42).Finally,we also tested the selectivity of the composite DCM-OH/MoS2 against other proteins,and found that it showed better selectivity for Aβ.The results demonstrate that the developed system shows certain biological application prospects.